Luc Reporter Cell Line-A549 is a polyclonal population which is constructed by lentivirus transduction, followed by stable cell selection. This cell line stably expresses luciferase gene and puromycin resistance gene driven by spleen focus-forming virus (SFFV) promoter. It is an ideal positive control for use in bioluminescence assays. In addition, A549 cells can form tumors in mouse lungs. The in vivo growth of these tumors can be monitored using bioluminescent imaging.