Luciferase Reporter Cell Line - Molm13

The researchers investigated how maternally expressed gene 3 (MEG3) combats chemoresistance in acute myeloid leukemia (AML). They discovered that MEG3 and microRNA (miR)-493-5p are downregulated in resistant AML cells when compared to parental cells. MEG3 increases miR-493-5p levels, which target methyltransferase-like 3 (METTL3). METTL3, in turn, stimulates MYC expression by increasing its m6A alteration. MEG3 and miR-493-5p overexpression, as well as METTL3 deletion, decreased cell growth and increased apoptosis in HL-60 and Molm13 cell lines, respectively. MEG3 also boosted AML cells' sensitivity to arabinocytosine (AraC). Suppressing miR-493-5p reversed the effects of MEG3. These data indicate that MEG3 increases AML cell chemosensitivity by modulating the miR-493-5p/METTL3/MYC axis.

Figure 1. The researchers confirmed that METTL3 is a miR-493-5p target by doing dual luciferase reporter tests with the MOLM13 cell line. They measured METTL3 expression in distinct AML and normal samples and discovered substantial differences. (Wang A, et al., 2022)