Luciferase Reporter Cell Line - MFE-280

MFE-280 is a human endometrial carcinoma cell line originally derived from a patient with endometrial adenocarcinoma. This cell line exhibits epithelial morphology and grows under adherent culture conditions. MFE-280 cells harbor specific genetic mutations and express distinct biomarkers, making them a valuable model for studying the biology of endometrial carcinoma, its hormonal responsiveness, and potential therapeutic interventions. These cells are known to express both estrogen receptors and progesterone receptors, rendering them particularly suitable for investigating the hormonal regulation involved in the progression of endometrial carcinoma. The MFE-280 Luciferase Reporter Cell Line is an engineered derivative of the parental MFE-280 cell line, designed to stably express the firefly luciferase reporter gene. This genetic modification enables the cells to constitutively produce luciferase—an enzyme that catalyzes the oxidation of a luciferin substrate, thereby generating a bioluminescent signal. The luciferase gene is stably integrated into the MFE-280 genome, ensuring the continuous and stable expression of the reporter protein across successive cell passages and providing researchers with a reliable and reproducible bioluminescent tool.

The MFE-280 Luciferase Reporter Cell Line serves as a versatile and powerful tool for a wide range of biomedical research applications, particularly in the fields of oncology, drug discovery, and molecular pharmacology. One of the primary applications of this cell line is in the screening of anti-cancer drugs and the assessment of therapeutic efficacy. In this context, the bioluminescent signal correlates directly with the number of viable cells, thereby enabling high-throughput screening of potential therapeutic compounds targeting endometrial carcinoma cells. Researchers can utilize MFE-280-Luc cells to quantitatively and efficiently evaluate the cytotoxicity, cytostatic effects, and anti-proliferative activity of novel drug candidates. This luciferase reporter system allows for the real-time monitoring of cell viability and proliferation dynamics without the need for cell lysis or the addition of toxic reagents, making it an ideal choice for longitudinal studies and the analysis of drug response kinetics. Furthermore, this reporter cell line has been widely employed in xenograft mouse models for in vivo bioluminescence imaging, where the engineered cells enable the non-invasive tracking of tumor establishment, growth, progression, and metastasis within living animals.