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  Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
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  Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
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  Advanced VLPs for vaccine development (Chikungunya, Dengue, SARS-CoV-2), gene therapy (AAV1 & AAV9), and drug screening (SSTR2, CCR5).
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  Accelerate your research with cost-effective LncRNA qPCR Array Technology.
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  Human Druggable Genome siRNA Library enables efficient drug target screening.
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  Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
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  Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
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  Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
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- Membrane Proteome Chip Antibody Off-Target Screening Service
  Membrane Proteome Chip Antibody Off‑Target Screening
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  Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
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CDMO
- HEK293 Cell-Based Production Platform
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  Stable expression over 15 generations with rapid cell line development in just 3 months.
  Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
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  Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
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  AI-Driven Gene Editing and Therapy
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  Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
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  Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
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Virology

protocols

Protocol

Protocol of Simplify the Adeno-Associated Virus Production Using Suspension HEK293T Cells

Experiment Tags: AAV (AAV), Suspension Cells, Serum-Free Media, Transfection Reagent, Lodixanol Density Gradient

Experiment Summary: In this protocol, we developed a simple scalable AAV production protocol using serum-free-media-adapted HEK293T suspension cells and VirusGEN transfection reagent.

protocols

Protocol

Modeling Hepatitis B Virus Infection in Non-Hepatic 293T-NE-3NRs Cells

Experiment Tags: HBV Infection Model, cell culture, HepG2, Co-culture

Experiment Summary: Current HBV research primarily focuses on hepatocytes, limiting insights into HBV-related pathology in non-hepatic tissues. This study introduces a protocol using non-hepatic 293T and hepatoma cells to investigate HBV infection. Two HBV infection methods were developed based on 293T-NE-3NRs.

protocols

Protocol

Ex Vivo Infection of Live Tissue with Oncolytic Viruses

Experiment Tags: Oncolytic viruses, tissue processing, viral infection, fluorescence microscopy, luciferase expression, plaque assay

Experiment Summary: As cancer treatments, oncolytic viruses (OVs) have potential, and determining whether living tissue specimens are infectious ex vivo prior to treatment offers a distinct benefit. This protocol describes the steps involved in preparing tissues for oncolytic virus ex vivo infection and the subsequent measurement of viral activity.

protocols

Protocol

Monkeypox Antigen Expression and Antibody Response Protocol

Experiment Tags: MPXV, A29L protein, B6R protein, M1R protein, HEK293T cells, Recombinant proteins, Protein purification, SDS-PAGE, Western blot, ELISA, Neutralization assay, Antibody titer

Experiment Summary: This protocol outlines the process for expressing, purifying, and analyzing MPXV antigens and assessing their immunogenicity, which is vital for vaccine research and development.

protocols

Protocol

Lentivirus Packaging Protocol

Experiment Tags: Lentivirus packaging protocol, lentivirus packaging, lentivirus, lentivirus production

Experiment Summary: Lentiviral packaging is a key method in molecular biology for delivering genetic material to target cells. By utilizing recombinant lentiviral particles, this protocol allows for highly efficient gene transfer, which is critical for research and therapeutic applications. This review discusses detailed lentiviral packaging protocols and highlights best practices for achieving optimal viral titers.

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