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Clones
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Experiment Tags: Lentiviral Transduction, Mouse Induced Pluripotent Stem Cells.
Experiment Summary: This protocol describes a method for generating and characterizing iPS cells from mouse embryonic fibroblasts using a lentiviral transduction system.
Experiment Tags: AAV Vectors, Intracranial Stereotactic Injection, AAV Delivery
Experiment Summary: This protocol describes how to perform an aseptic stereotaxic survival surgery to deliver AAV vectors to the brain of a mouse.
Experiment Tags: Flavivirus, Fluorescence, NS2B-NS3 (NS2B-NS3), Live-cell imaging, Reporter cells, Plaque assay, Image analysis
Experiment Summary: This protocol describes methods for generating, selecting and implementing stable BHK-21 cells that express molecular reporters encoding viral genes suitable for monitoring viral infection by live cell imaging.
Experiment Tags: Cell Immortalization, Lentiviral Transduction
Experiment Summary: The protocol introduces the definition of cell immortalization and commonly used viral transfection methods, and describes in detail the steps to generate immortalized cells using prefabricated cell immortalization lentiviral particles.
Experiment Tags: CRISPR, Cpf1, AAV, CAR-T, T Cell, Cancer, Immunotherapy
Experiment Summary: This protocol describes a new method, the AAV-Cpf1 KIKO system, using a combination of viral and non-viral approaches to generate a stable CAR-T with homology-directed repair (HDR) knock-in and immune checkpoint knockout at high efficiency in one step.
Experiment Tags: NLR, NOD1, Lentivirus, Stable Expression, TRE, Tetracycline
Experiment Summary: In this protocol, we describe a method for stable, conditional expression of Nod-Like receptors (NLRs) in THP-1 cells using a lentiviral expression system.
Experiment Tags: Ex vivo tissue culture model, Lentiviral, Proliferation, Oncogenic , Transformation, Prostate
Experiment Summary: This protocol describes how to generate lentiviral vectors and particules, successfully transduce human prostate tissues, induce exogenous gene expression, and assess cellular proliferation.
Experiment Tags: Lentivirus, Stable cell lines, Gene knockdown, Gene over-expression, Mammalian cell lines, Transduction
Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.
Experiment Tags: Neuron-preferential, Lentiviral vectors
Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.
Experiment Tags: Virus, Cryopreservation
Experiment Summary: This experiment describes the method for rapid cryopreservation of viruses.
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