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Virology

protocols
Protocol

Virus Cryopreservation Technology

Experiment Tags: Virus, Cryopreservation

Experiment Summary: This experiment describes the method for rapid cryopreservation of viruses.

protocols
Protocol

Production of Neuron-Preferential Lentiviral Vectors Protocol

Experiment Tags: Neuron-preferential, Lentiviral vectors

Experiment Summary: This protocol describes a procedure for the direct injection of adenovirus into the CNS using stereotaxic guidance.

protocols
Protocol

Protocol for Generating Stably Expressed Mammalian Cell Lines Using Lentivirus

Experiment Tags: Lentivirus, Stable cell lines, Gene knockdown, Gene over-expression, Mammalian cell lines, Transduction

Experiment Summary: This protocol describes the detailed steps for generating knockout cell lines (coding or non-coding regions) using CRISPR/Cas9 tools.

protocols
Protocol

Ex vivo Culture and Lentiviral Transduction Protocol of Benign Prostatic Hyperplasia (BPH) Samples

Experiment Tags: Ex vivo tissue culture model, Lentiviral, Proliferation, Oncogenic, Transformation, Prostate

Experiment Summary: This protocol describes efficient delivery of AAV to mediate GFP or Cre expression for cell lineage tracing in the mouse pancreas or for the delivery of transgenes under a specific promoter to these cells.

protocols
Protocol

Lentivirus-mediated Conditional Gene Expression Protocol

Experiment Tags: NLR, NOD1, Lentivirus, Stable Expression, TRE, Tetracycline

Experiment Summary: This protocol is to be used to test the binding and activity of miRNA on putative UTR target sequences. It is based on the expression of Luciferase as a reporter gene fused to the UTR sequence in the presence of plasmids containing pre-miRNA of interest or synthetic miRNA to test in an in vitro cell culture assay.