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Virology

protocols
Protocol

Purification and Injection of Retroviral Vectors Protocol

Experiment Tags: Retroviral Vectors, Purification, Injection.

Experiment Summary: In this protocol, we detail two different methods of detecting viral RNA synthesis: a firefly luciferase assay for rapid and sensitive measurement of RSV polymerase activity; and a real-time quantitative PCR method for determination of specific effects on the transcription of individual viral genes and the polar transcription gradient of RSV.

protocols
Protocol

In Vivo Adenovirus-Mediated Gene Transfer into the CNS of Adult Rats Protocol

Experiment Tags: Gene Transfer, Central Nervous System (CNS), Adenoviral Vectors

Experiment Summary: This protocol describes how to generate a single guide RNA (sgRNA) library for use in genetic screening.

protocols
Protocol

Simplified Purification of AAV and Delivery to the Pancreas by Intraductal Administration Protocol

Experiment Tags: Adeno-Associated Virus (AAV), PEG Aqua Partitioning, AAV Viral Purification, Pancreatic Ductal Infusion, Pancreatic Cell Lineage Tracing

Experiment Summary: This protocol evaluates in detail the effect of SMase treatment on RuV infectivity in RK13 and Vero cells.

protocols
Protocol

Glioma Induction by Intracerebral Retrovirus Injection Protocol

Experiment Tags: Glioma, Retrovirus, Intracerebral Injection, Murine Model, PDGFBB(PDGFBB), PTEN(PTEN), P53(P53)

Experiment Summary: In this protocol, we describe the transduction of cultured cells with recombinant lentivirus expressing fluorescent markers.

protocols
Protocol

Protocol for Analyzing of the Effect of Sphingomyelinase on Rubella Virus Infectivity in Two Cell Lines

Experiment Tags: Rubella Virus, Lipid, Sphingomyelin, Virus Entry, Sphingomyelinase

Experiment Summary: This protocol describes how to generate lentiviral vectors and particules, successfully transduce human prostate tissues, induce exogenous gene expression, and assess cellular proliferation.