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Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
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Clones
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Kits
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Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
Enzymes
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Powerful Tn5 Transposase for DNA insertion and random library construction.
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Experiment Tags: Influenza A virus, Infected mouse model, H1N1 (H1N1), Virus production, Plaque assay
Experiment Summary: In this protocol, we report a protocol in infected mice with different virus doses and strains to explore how an inhibitor of lysine-specific demethylase (LSD1) impacts disease progression.
Experiment Tags: Respiratory Syncytial Virus, Respiratory Infection, Virus Detection
Experiment Summary: In this protocol, we discuss in detail how to infect BALB/c mice with RSV and how to detect the RSV genome in mouse lungs using reverse transcription quantitative PCR (RT-qPCR).
Experiment Tags: Zika Virus, NS4B (NS4B), Double-Stranded RNA, Virus Titration, Immunofluorescence Microscopy, Plaque Assay
Experiment Summary: In this protocol, we describe basic experimental procedures to produce ZIKV stocks and to quantify their concentration in infectious virus particles as well as to image and study this pathogen within infected cells using confocal microscopy-based imaging.
Experiment Tags: Chikungunya, Enzyme-Linked Immunosorbent Assay (ELISA)
Experiment Summary: In this protocol, we accurately describe specific assays that can deterministically detect anti-chikungunya virus antibodies (Ab) in the tissues or sera of infected mice.
Experiment Tags: Biological Techniques, Recombinant, Lentivirus, Transduction
Experiment Summary: In this protocol, we describe the transduction of cultured cells with recombinant lentivirus expressing fluorescent markers.
Experiment Tags: Rubella Virus, Lipid, Sphingomyelin, Virus Entry, Sphingomyelinase
Experiment Summary: This protocol evaluates in detail the effect of SMase treatment on RuV infectivity in RK13 and Vero cells.
Experiment Tags: Glioma, Retrovirus, Intracerebral Injection, Murine Model, PDGFBB(PDGFBB), PTEN(PTEN), P53(P53)
Experiment Summary: This protocol describes a simple and practical method for inducing GBM formation by retrovirus injection in the murine brain. This system gives a spatial and temporal control over the induction of glioma and allows the assessment of therapeutic effects with a bioluminescent reporter.
Experiment Tags: Adeno-Associated Virus (AAV), PEG Aqua Partitioning, AAV Viral Purification, Pancreatic Ductal Infusion, Pancreatic Cell Lineage Tracing
Experiment Summary: This protocol describes efficient delivery of AAV to mediate GFP or Cre expression for cell lineage tracing in the mouse pancreas or for the delivery of transgenes under a specific promoter to these cells.
Experiment Tags: Gene Transfer, Central Nervous System (CNS), Adenoviral Vectors
Experiment Summary: This protocol describes a procedure for the direct injection of adenovirus into the CNS using stereotaxic guidance.
Experiment Tags: Retroviral Vectors, Purification, Injection.
Experiment Summary: This protocol discusses the production, purification, and use of replication-deficient retroviral vectors based on Moloney murine leukemia virus and lentivirus. It also describes the injection of a retroviral vector into the dentate gyrus of young adult mice to fluorescently label live murine brain tissue.
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