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Clones
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Kits
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Experiment Tags: Virus, Titration, Infectivity, TCID5
Experiment Summary: This experiment presents a method for the titration of the infectivity of two viruses.
Experiment Tags: Lentivirus, Lentivirus Concentration, Lentivirus Purification
Experiment Summary: This experiment introduced the operation steps of concentrating and purifying lentivirus by ultracentrifugation precipitation method.
Experiment Tags: Lentivirus, Lentivirus Concentration, Lentivirus Purification
Experiment Summary: This experiment introduced the operation steps of concentrating and purifying lentivirus with PEG-8000 concentration method.
Experiment Tags: Adenovirus
Experiment Summary: In our protocol, the recombination between the shuttle plasmid and the adenovirus genome contained in the backbone plasmid is performed in the E. coli strain. Positive recombinants are selected by resistance to an antibiotic.
Experiment Tags: Adenovirus, Recombinant Adenovirus
Experiment Summary: Conventional approaches to producing small volumes of viral vectors involve culturing cells in stationary and adherent cultures.
Experiment Tags: Viral Titering, TCID50 Assay
Experiment Summary: A TCID50 was found of greatest value when lack of experimental material or costs of analysis precludes extensive replicate titer determinations but good estimates of titers and treatment differences are essential.
Experiment Tags: Viral Titering, Plaque Assay
Experiment Summary: After fixing and staining the infected cellular monolayer, plaques are counted so that titer viral stock samples in terms of plaque forming units (pfu) per milliliter.
Experiment Tags: Lentivirus packaging protocol, lentivirus packaging, lentivirus, lentivirus production
Experiment Summary: Lentiviral packaging is a key method in molecular biology for delivering genetic material to target cells. By utilizing recombinant lentiviral particles, this protocol allows for highly efficient gene transfer, which is critical for research and therapeutic applications. This review discusses detailed lentiviral packaging protocols and highlights best practices for achieving optimal viral titers.
Experiment Tags: Lentivirus, Lentivirus Transduction
Experiment Summary: Lentivirus is a modified HIV virus that must be handled with caution although it cannot replicate in a host.
Experiment Tags: Baculovirus, Baculovirus expression vector system, BEVS
Experiment Summary: BEVS is recognized as a powerful tool for the expression of the large-scale protein on the strength of convenient process, high expression level and the function of post-translational modifications.
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