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Virology

protocols
Protocol

Rapid Modular CAR-T Generation with CRISPR/Cpf1 and AAV Systems Protocol

Experiment Tags: CRISPR, Cpf1, AAV, CAR-T, T Cell, Cancer, Immunotherapy

Experiment Summary: In this detailed protocol, the circRNA of interest (e.g., circHipk2) was captured using a biotin-labeled antisense oligo (ASO) targeting the circHipk2 backsplice junction sequence followed by pulldown with streptavidin-conjugated magnetic beads. The specific enrichment of circRNA was analyzed using reverse transcription quantitative PCR (RT-qPCR).

protocols
Protocol

Cell Immortalization Protocol Using Lentiviral Particles

Experiment Tags: Cell Immortalization, Lentiviral Transduction

Experiment Summary: This protocol describes a simple and straightforward way to obtain single-stranded circular RNA sequences in vitro.

protocols
Protocol

Generation and Implementation of Reporter BHK-21 Cells for Live Imaging of Flavivirus Infection Protocol

Experiment Tags: Flavivirus, Fluorescence, NS2B-NS3 (NS2B-NS3), Live-cell imaging, Reporter cells, Plaque assay, Image analysis

Experiment Summary: In this protocol, we describe a quantitative PCR (qPCR) based method to determine the amount of vector bound to the cell surface and the amount of subsequent virus internalization based on viral genome quantification.

protocols
Protocol

Intracranial Stereotactic Injection of Adeno-Associated Viral Vectors Protocol

Experiment Tags: AAV Vectors, Intracranial Stereotactic Injection, AAV Delivery

Experiment Summary: In this article, we describe the application of ATAC-seq in zebrafish embryos, thus illustrating the applicability of this method to the entire vertebrate population.

protocols
Protocol

Protocol for Generating Mouse Induced Pluripotent Stem Cells by Lentiviral Transduction

Experiment Tags: Lentiviral Transduction, Mouse Induced Pluripotent Stem Cells.

Experiment Summary: In this protocol, we briefly describe the CRISPR/Cas9 library screening process to simplify and facilitate high-throughput screening.