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Virology

protocols
Protocol

Transduction of Cultured Cells with Recombinant Lentiviral Particles Protocol

Experiment Tags: Biological Techniques, Recombinant, Lentivirus, Transduction

Experiment Summary: This protocol might work for searching complementation of other gene deletion mutants that have a good selective phenotype.

protocols
Protocol

Chikungunya ELISA Protocol

Experiment Tags: Chikungunya, Enzyme-Linked Immunosorbent Assay (ELISA)

Experiment Summary: This protocol describes methods for generating, selecting and implementing stable BHK-21 cells that express molecular reporters encoding viral genes suitable for monitoring viral infection by live cell imaging.

protocols
Protocol

Protocol for The Production, Titration and Imaging of Zika Virus in Mammalian Cells

Experiment Tags: Zika Virus, NS4B (NS4B), Double-Stranded RNA, Virus Titration, Immunofluorescence Microscopy, Plaque Assay

Experiment Summary: This protocol describes how to perform an aseptic stereotaxic survival surgery to deliver AAV vectors to the brain of a mouse.

protocols
Protocol

Protocol on Respiratory Syncytial Virus Infection in Mice and Detection of Viral Genomes in the Lung Using RT-qPCR

Experiment Tags: Respiratory Syncytial Virus, Respiratory Infection, Virus Detection

Experiment Summary: This protocol describes a method for generating and characterizing iPS cells from mouse embryonic fibroblasts using a lentiviral transduction system.

protocols
Protocol

H1N1 Virus Production and Infection Protocol

Experiment Tags: Influenza A virus, Infected mouse model, H1N1 (H1N1), Virus production, Plaque assay

Experiment Summary: This protocol describes the construction of a cDNA library from the model plant Arabidopsis and a procedure for screening interaction proteins of AtSR1/CAMTA3, a Ca2+/CaM-regulated transcription factor from this library.