Transfected Stable Cell Lines
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Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Premade Virus Particles
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Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
Virus-Like Particles (VLPs)
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Advanced VLPs for vaccine development (Chikungunya, Dengue, SARS-CoV-2), gene therapy (AAV1 & AAV9), and drug screening (SSTR2, CCR5).
Oligonucleotide Products
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Accelerate your research with cost-effective LncRNA qPCR Array Technology.
RNA Interference Products
Targeted | Potent | High Specificity
Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
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Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Clones
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Ready-to-use clones for streamlined research and development.
Kits
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Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
Enzymes
Purified | Stable | Efficient
Powerful Tn5 Transposase for DNA insertion and random library construction.
Aptamers
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Aptamers for key proteins like ACVR1A, Akt, EGFR, and VEGFR.
Adjuvants
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Enhance immune responses with high-purity, potent CpG ODNs.
Laboratory Equipment
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Effortlessly streamline DNA extraction with CB™ Magnetic-Nanoparticle Systems.
Stable Cell Line Generation
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Fast proposals, regular updates, and detailed reports; strict quality control, and contamination-free cells; knockout results in 4-6 weeks.
Target-based Drug Discovery Service
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Target identification, validation, and screening for drug discovery and therapeutic development.
Custom Viral Service
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Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
Custom Antibody Service
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End-to-end antibody development support, from target to validation, enabling clients to rapidly obtain application-ready antibodies.
Antibody-Drug Conjugation Service
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Comprehensive solutions covering design, development, and validation to ensure conjugated drugs with consistent quality and clinical potential.
Protein Degrader Service
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Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
Nucleotides Service
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Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
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RNA design, synthesis, and manufacturing—covering mRNA, saRNA, circRNA, and RNAi. Fast turnaround, rigorous QC, and seamless transition from research to GMP production.
Custom Libraries Construction Service
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Custom cDNA, genomic, and mutagenesis libraries for drug discovery, screening, and functional genomics.
Gene Editing Services
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Gene editing solutions for gene editing, knockouts, knock-ins, and customized genetic modifications. Integrated multi-platform solutions for one-stop CRISPR sgRNA library synthesis and gene screening services
Microbe Genome Editing Service
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Enhance microbial productivity with advanced genome editing using Rec-mediated recombination and CRISPR/Cas9 technologies.
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Complete biosafety testing solutions for gene therapy, viral vectors, and biologics development.
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Genetic modification for crop improvement, biotechnology, and plant-based research solutions.
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Plant-based protein expression systems for biopharmaceuticals, enzyme production, and research.
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Revolutionizing drug delivery and diagnostic development with next-generation high-throughput aptamer selection and synthesis technologies.
CGT Biosafety Testing
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Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
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Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
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Scalable mRNA production from milligrams to grams, with personalized process design for sequence optimization, cap selection, and nucleotide modifications, all in one service.
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Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
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Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
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Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
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Experiment Tags: Lentiviral Vectors, Stable Cell Line, Gene Expression, Viral Transduction, Cell Culture, Puromycin Selection, Virus Packaging, Mammalian Cells, Transgene Expression
Experiment Summary: This protocol details the generation of stable cell lines using lentiviral vectors. The method covers plasmid preparation, 293T cell transfection for viral packaging, PEG8000-based virus concentration, target cell infection, antibiotic selection parameters, and single-clone isolation. Includes MOI calculation guidance, troubleshooting for common challenges like low viral titer and poor infection efficiency, and validation strategies for ensuring stable transgene expression.
Experiment Tags: MPXV, A29L protein, B6R protein, M1R protein, HEK293T cells, Recombinant proteins, Protein purification, SDS-PAGE, Western blot, ELISA, Neutralization assay, Antibody titer
Experiment Summary: This protocol outlines the process for expressing, purifying, and analyzing MPXV antigens and assessing their immunogenicity, which is vital for vaccine research and development.
Experiment Tags: Oncolytic viruses, tissue processing, viral infection, fluorescence microscopy, luciferase expression, plaque assay
Experiment Summary: As cancer treatments, oncolytic viruses (OVs) have potential, and determining whether living tissue specimens are infectious ex vivo prior to treatment offers a distinct benefit. This protocol describes the steps involved in preparing tissues for oncolytic virus ex vivo infection and the subsequent measurement of viral activity.
Experiment Tags: HBV Infection Model, cell culture, HepG2, Co-culture
Experiment Summary: Current HBV research primarily focuses on hepatocytes, limiting insights into HBV-related pathology in non-hepatic tissues. This study introduces a protocol using non-hepatic 293T and hepatoma cells to investigate HBV infection. Two HBV infection methods were developed based on 293T-NE-3NRs.
Experiment Tags: AAV (AAV), Suspension Cells, Serum-Free Media, Transfection Reagent, Lodixanol Density Gradient
Experiment Summary: In this protocol, we developed a simple scalable AAV production protocol using serum-free-media-adapted HEK293T suspension cells and VirusGEN transfection reagent.
Experiment Tags: Retrovirus, Enveloped Virus, MLV, HIV, Capsid Core Stability Assay, Fate of Capsid Assay, Glycosylated Gag, gPr80, TRIM5α
Experiment Summary: In this protocol, we describe a method using an in vitro detergent-based approach combined with ultracentrifugation for assessing viral capsid core stability. The results show that this method is a significantly simpler and faster way to assess relative capsid core stability.
Experiment Tags: Lentiviral Transduction, CAR T cells
Experiment Summary: In this protocol, an easy and reliable approach for CAR T cell generation is described. T cells are first isolated from peripheral blood and afterwards activated for one day with anti-CD3/CD28 Dynabeads. The gene transfer is performed by lentiviral transduction and gene transfer rate can be verified by flowcytometric analysis.
Experiment Tags: Varicella Zoster Virus (VZV), Replication Assays
Experiment Summary: This protocol describes a basic VZV replication assay using a recombinant VZV-GFP reporter virus. This reporter virus-infected cell line can be used in combination with siRNA gene depletion or cDNA overexpression transfection protocols to determine the effect of individual cellular genes on virus replication.
Experiment Tags: Aedes aegypti, Mosquito, Zika virus, Arbovirus, Intrathoracic Inoculation, Microinjection
Experiment Summary: This protocol describes herein a protocol for the intrathoracic inoculation of ZIKV in Ae. aegypti. This method bypasses the midgut, which leads to a more rapid and higher proportion of disseminated infections in comparison to oral challenge, and mosquitoes become infected with a consistent dose of virus.
Experiment Tags: Adeno-Associated Viruses, Acute Brain Slices, Genetically-Encoded Fluorescent Biosensors, Hippocampus, Intracranial Injection, Dentate Granule cells
Experiment Summary: In this protocol, we describe a protocol for intracranial injections of two viral vectors encoding the metabolic biosensor Peredox and the calcium biosensor RCaMP1h. When combined with 2-photon microscopy and fluorescence lifetime imaging, this protocol allows the simultaneous quantitative assessment of changes in the cytosolic NADH/NAD+ ratio and the intracellular Ca2+ levels in individual dentate granule cells from acute hippocampal slices.
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