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Protocols

protocols
Protocol

PCR Amplification Preparation with Labeled Probes Protocol

Experiment Tags: PCR Amplification, Probe Preparation

Experiment Summary: This experiment introduces the PCR labeling technique of probes using biotin and digoxin as examples.

protocols
Protocol

Protocol for Detection of Cytoplasmic and Nuclear Circular RNA via RT-qPCR

Experiment Tags: Subcellular Fractionation, RT-qPCR, Circular RNA, circRNA, Cytoplasmic circRNA, Nuclear circRNA, Low-Abundance circRNA

Experiment Summary: In this protocol, we describe a method for stable, conditional expression of Nod-Like receptors (NLRs) in THP-1 cells using a lentiviral expression system.

protocols
Protocol

Bacterial Total Protein and Membrane Protein Extraction Protocol

Experiment Tags: Membrane Protein, Bacterial Total Protein, Protein Extract

Experiment Summary: This experiment describes several methods for extracting total bacterial proteins and membrane proteins.

protocols
Protocol

What is the ELISpot Assay? Complete Guide to Principle, Steps, and Uses

Experiment Tags: ELISpot Assay, Cytokine Detection, Single-Cell Immunoassay

Experiment Summary: The ELISpot (Enzyme-Linked ImmunoSpot) assay is a highly sensitive immunological technique that detects secreted proteins—such as cytokines or antibodies—at the single-cell level. It quantifies the frequency of secreting cells, offering functional insights that conventional ELISA cannot provide.

protocols
Protocol

Baculovirus Expression Protocol

Experiment Tags: Baculovirus, Baculovirus expression vector system, BEVS

Experiment Summary: BEVS is recognized as a powerful tool for the expression of the large-scale protein on the strength of convenient process, high expression level and the function of post-translational modifications.