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Experiment Tags: Neuron-preferential, Lentiviral vectors
Experiment Summary: This protocol describes a procedure for the direct injection of adenovirus into the CNS using stereotaxic guidance.
Experiment Tags: Lentivirus, Stable cell lines, Gene knockdown, Gene over-expression, Mammalian cell lines, Transduction
Experiment Summary: This protocol describes the detailed steps for generating knockout cell lines (coding or non-coding regions) using CRISPR/Cas9 tools.
Experiment Tags: Ex vivo tissue culture model, Lentiviral, Proliferation, Oncogenic, Transformation, Prostate
Experiment Summary: This protocol describes efficient delivery of AAV to mediate GFP or Cre expression for cell lineage tracing in the mouse pancreas or for the delivery of transgenes under a specific promoter to these cells.
Experiment Tags: NLR, NOD1, Lentivirus, Stable Expression, TRE, Tetracycline
Experiment Summary: This protocol is to be used to test the binding and activity of miRNA on putative UTR target sequences. It is based on the expression of Luciferase as a reporter gene fused to the UTR sequence in the presence of plasmids containing pre-miRNA of interest or synthetic miRNA to test in an in vitro cell culture assay.
Experiment Tags: CRISPR, Cpf1, AAV, CAR-T, T Cell, Cancer, Immunotherapy
Experiment Summary: In this detailed protocol, the circRNA of interest (e.g., circHipk2) was captured using a biotin-labeled antisense oligo (ASO) targeting the circHipk2 backsplice junction sequence followed by pulldown with streptavidin-conjugated magnetic beads. The specific enrichment of circRNA was analyzed using reverse transcription quantitative PCR (RT-qPCR).
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