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Protocols

protocols
Protocol

Chikungunya ELISA Protocol

Experiment Tags: Chikungunya, Enzyme-Linked Immunosorbent Assay (ELISA)

Experiment Summary: This protocol describes methods for generating, selecting and implementing stable BHK-21 cells that express molecular reporters encoding viral genes suitable for monitoring viral infection by live cell imaging.

protocols
Protocol

Protocol for The Production, Titration and Imaging of Zika Virus in Mammalian Cells

Experiment Tags: Zika Virus, NS4B (NS4B), Double-Stranded RNA, Virus Titration, Immunofluorescence Microscopy, Plaque Assay

Experiment Summary: This protocol describes how to perform an aseptic stereotaxic survival surgery to deliver AAV vectors to the brain of a mouse.

protocols
Protocol

Protocol on Respiratory Syncytial Virus Infection in Mice and Detection of Viral Genomes in the Lung Using RT-qPCR

Experiment Tags: Respiratory Syncytial Virus, Respiratory Infection, Virus Detection

Experiment Summary: This protocol describes a method for generating and characterizing iPS cells from mouse embryonic fibroblasts using a lentiviral transduction system.

protocols
Protocol

H1N1 Virus Production and Infection Protocol

Experiment Tags: Influenza A virus, Infected mouse model, H1N1 (H1N1), Virus production, Plaque assay

Experiment Summary: This protocol describes the construction of a cDNA library from the model plant Arabidopsis and a procedure for screening interaction proteins of AtSR1/CAMTA3, a Ca2+/CaM-regulated transcription factor from this library.

protocols
Protocol

Protocol for Delivering AAV to Express Fluorescent Biosensors in the Juvenile Mouse Hippocampus

Experiment Tags: Adeno-Associated Viruses, Acute Brain Slices, Genetically-Encoded Fluorescent Biosensors, Hippocampus, Intracranial Injection, Dentate Granule cells

Experiment Summary: In this protocol, we describe a protocol for intracranial injections of two viral vectors encoding the metabolic biosensor Peredox and the calcium biosensor RCaMP1h. When combined with 2-photon microscopy and fluorescence lifetime imaging, this protocol allows the simultaneous quantitative assessment of changes in the cytosolic NADH/NAD+ ratio and the intracellular Ca2+ levels in individual dentate granule cells from acute hippocampal slices.