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Protocols

protocols
Protocol

Fosmid Library Construction Protocol

Experiment Tags: Fosmid Library, Library, Library Construction

Experiment Summary: The fosmid system has been widely used in the construction of large-insert genomic libraries. Fosmid clones are similar in insert size comparing with cosmid clones and smaller than that in BAC and yeast artificial chromosomes (YAC).

protocols
Protocol

cDNA Library Construction Protocol

Experiment Tags: cDNA Library, Library, Library Construction

Experiment Summary: To create a cDNA library, these mRNA molecules are treated with the enzyme reverse transcriptase, which is used to make a DNA copy of an mRNA (i.e., cDNA).

protocols
Protocol

BCA Library Construction Protocol

Experiment Tags: BCA Library, Library, Library Construction

Experiment Summary: The size-fractionated DNA is cloned into a BAC vector and transformed into E. coli by electrical shock. The transformants are arrayed into microtiter dishes and high-density replica filters are prepared to facilitate screening of the library.

protocols
Protocol

Labeling Protocol for Microarray Analysis

Experiment Tags: Labeling, Microarray Analysis

Experiment Summary: This experiment describes how to perform labeling procedure for microarray analysis.

protocols
Protocol

Preparation of Genomic Overexpression Library Protocol

Experiment Tags: Genomic, Overexpression, Library

Experiment Summary: This protocol describes a simple and practical method for inducing GBM formation by retrovirus injection in the murine brain. This system gives a spatial and temporal control over the induction of glioma and allows the assessment of therapeutic effects with a bioluminescent reporter.