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Bioinformatics

protocols
Protocol

Preparation of Genomic Overexpression Library Protocol

Experiment Tags: Genomic, Overexpression, Library

Experiment Summary: This protocol describes a simple and practical method for inducing GBM formation by retrovirus injection in the murine brain. This system gives a spatial and temporal control over the induction of glioma and allows the assessment of therapeutic effects with a bioluminescent reporter.

protocols
Protocol

Protocol for Constructing Glycine Oxidase Mutant Libraries by Random Mutagenesis

Experiment Tags: Glycine Oxidase Mutant Libraries, Random Mutagenesis

Experiment Summary: In this protocol, we describe a simple and widely used <em>in vitro</em> transcription reaction for the preparation of double-stranded RNA using PCR templates. The dsRNA prepared by this method can be used to induce RNA interference in certain cells or tissues.

protocols
Protocol

CRISPR/Cas9 Library Screening Protocol

Experiment Tags: CRISPRCas9 Library Screening, CRISPRCas9

Experiment Summary: In this protocol, we discuss in detail how to infect BALB/c mice with RSV and how to detect the RSV genome in mouse lungs using reverse transcription quantitative PCR (RT-qPCR).

protocols
Protocol

Single Guide RNA Library Design and Construction Protocol

Experiment Tags: Single Guide RNA Library, Library Design and Construction

Experiment Summary: This protocol describes the use of the 3rd generation lentiviral system which uses three different plasmids for generating stable cell lines.