Syn-GFP Lentivirus (Cre Dependent)

Viral vectors are extremely important tools for transducing functional genes into target tissue cells in gene therapy. The modification, recombination and optimization of viral vectors are important foundations for gene therapy engineering. At present, the more mature viral vectors include retrovirus, adenovirus vectors and related viral vectors. With the expansion and deepening of various virus research fields, the characteristics and good natural advantages of some other viruses are gradually recognized by people. Among them, viral vectors represented by lentivirus and herpes stomatitis virus have gradually become research hotspots with their unique advantages and good vector characteristics. Driven by genetic engineering technology, it is expected to become a new generation of ideal viral vectors. Lentivirus represented by HIV-1 can act on the G0/G1 phase of the cell cycle and has nucleophilicity, so it can infect non-dividing and dividing cells. Studies have shown that lentiviral vectors also have the advantages of large capacity of transferred gene fragments, target genes can be integrated into the target cell genome for long-term expression, and small immune response. At the same time, lentiviral vectors have excellent cell tropism for hematopoietic stem cells, immune cells, nerve cells, hepatocytes, cardiomyocytes, muscle cells, retinal cells and other types of cells, which are very important targets for gene therapy. Therefore, lentiviral vectors have natural advantages in becoming genetic engineering vectors.