GFP Reporter Cell Line - SNU-5

REH cells are a widely studied human cell line used in cancer research, particularly leukemia research. They were derived from the peripheral blood of a 15-year-old female patient diagnosed with acute lymphoblastic leukemia (ALL), the most common cancer in children. The cells exhibit morphology typically associated with lymphoblasts, a type of immature white blood cell. Researchers appreciate the fact that REH cells proliferate rapidly, which makes them suitable for a variety of in vitro assays, including drug sensitivity testing, gene expression studies, and cytotoxicity assays. GFP/Luc Reporter Cell Line - REH is genetically engineered to express two important reporter genes: green fluorescent protein (GFP) and luciferase (Luc). This dual reporter system enables researchers to monitor and quantify cellular processes with high sensitivity and specificity. GFP is a protein derived from the jellyfish Aequorea victoria that emits bright green fluorescence when exposed to blue to ultraviolet light. Its expression in REH cells allows for real-time visualization of cellular events by fluorescence microscopy or flow cytometry. This fluorescent marker provides a convenient and non-invasive way to track cell proliferation, differentiation, and localization in live cell imaging experiments. Luciferase is an enzyme that catalyzes a bioluminescent reaction, producing light in the presence of its substrate, luciferin. Expression of the Luc gene in REH cells facilitates highly sensitive detection and quantification of cellular activities, such as gene expression and metabolic processes, through bioluminescent imaging. This allows for precise measurements in both in vitro assays and in vivo studies using animal models.