GFP Reporter Cell Line - MM1.R

Name: GFP Reporter Cell Line - MM1.R
SKU: CSC-RR0553
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0553

Host Cell : MM1.R Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RR0553
Description	This cell line is engineered to stably express GFP reporter gene in MM1.R.
Target Gene	GFP
Host Cell	MM1.R
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Quick Inquiry

Background

Applications

Q & A

Customer Reviews

The MM1.R cell line is a human multiple myeloma (MM) cell line that has been engineered to express the GFP reporter gene. Multiple myeloma is a malignant disease characterized by the proliferation of plasma cells in the bone marrow, leading to bone destruction, anemia, and other complications. The stable expression of GFP in MM1.R cells allows for the visualization of myeloma cells, facilitating the study of their behavior and the effects of potential therapeutic interventions.

The GFP reporter cell line in MM1.R cells is a powerful tool for investigating the molecular mechanisms of myeloma progression, including the study of cell proliferation, survival, and drug resistance. It enables researchers to track myeloma cells in vitro and in animal models, providing insights into the complex interactions between tumor cells and their microenvironment. This cell line is also useful for evaluating the efficacy of novel therapeutic agents, as it allows for the direct observation of the effects of treatments on myeloma cells, which can inform the development of more effective strategies for the management of multiple myeloma.

The GFP Reporter Cell Line - MM1.R is a human myeloid leukemia cell line that expresses GFP. This cell line is particularly useful for studying the molecular mechanisms of leukemia and for evaluating potential anti-leukemic therapies.

(1)Leukemia Biology Research: The MM1.R GFP cells can be used to explore the biology of myeloid leukemia, including the characterization of leukemic stem cells and the investigation of molecular pathways involved in disease progression.

(2)Therapeutic Target Identification: The GFP reporter facilitates the identification of therapeutic targets in myeloid leukemia. By monitoring GFP expression in response to various treatments, researchers can screen for compounds that modulate cell viability and proliferation.

(3)Signaling Pathway Analysis: The MM1.R cell line can be employed to study intracellular signaling pathways that regulate cell growth, survival, and differentiation in myeloid leukemia. The GFP signal can be used to monitor the activation of specific pathways in response to different stimuli.

Customer Q&As

How can the GFP Reporter Cell Line - MM1.R be utilized to study the bone marrow microenvironment's influence on multiple myeloma progression?

A: Researchers can co-culture GFP-labeled MM1.R cells with bone marrow stromal cells or within a bone marrow mimicking matrix to observe interactions and effects on cell proliferation, survival, and drug resistance. The GFP fluorescence facilitates tracking and quantification of myeloma cells under various microenvironmental conditions.

What advantages does the GFP Reporter Cell Line - MM1.R offer for evaluating the efficacy of CAR-T cell therapy against multiple myeloma in preclinical models?

A: The GFP-labeled MM1.R cells allow for real-time tracking of myeloma cells in vitro and in vivo, enabling researchers to directly observe the targeting and eradication of these cells by CAR-T therapy. This cell line allows for the quantification of CAR-T cell-mediated cytotoxicity and the assessment of therapeutic efficacy over time.

Can the GFP Reporter Cell Line - MM1.R be employed to investigate the mechanisms of drug resistance in multiple myeloma?

A: Yes, the cell line can be exposed to various anti-myeloma drugs to develop resistant cell populations. GFP fluorescence can then be used to isolate surviving cells for further analysis, such as gene expression profiling and signaling pathway studies, to elucidate the mechanisms underlying drug resistance.

How might researchers use the GFP Reporter Cell Line - MM1.R to explore the role of specific genes in multiple myeloma cell migration and invasion?

A: Gene editing tools, like CRISPR/Cas9, can be applied to GFP-labeled MM1.R cells to knock out or overexpress target genes. The modified cells can then be analyzed using migration and invasion assays, with GFP fluorescence enabling easy visualization and quantification of cell movements.

In what ways could the GFP Reporter Cell Line - MM1.R facilitate the development and validation of imaging agents for the early detection of multiple myeloma?

A: The GFP fluorescence in MM1.R cells provides a means to test the sensitivity and specificity of novel imaging agents in recognizing and binding to myeloma cells. In vivo imaging of mouse models inoculated with GFP-labeled MM1.R cells can demonstrate the agents' ability to detect small numbers of myeloma cells in various tissues.

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Customer Reviews

Real-Time Monitoring

We can monitor gene expression in real-time, providing a dynamic view of cellular processes without the need for time-consuming endpoint assays.

United Kingdom

2022-11-21

Quantitative Analysis

The fluorescence intensity can be measured quantitatively, offering a straightforward method to assess changes in gene expression levels.

Germany

2020-06-14

Non-Invasive Assay

The use of GFP negates the need for invasive procedures or cell lysis, allowing for the study of living cells over extended periods.

Germany

2022-08-16

Visual Gene Expression

The GFP Reporter Cell Line - MM1.R allows for direct visualization of gene expression through the green fluorescence protein, simplifying and speeding up analysis.

French

2022-09-29

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GFP Reporter Cell Line - MM1.R

Cell Line Information

Cell Culture Information

Safety and Packaging

Background

Applications

Q & A

Customer Reviews

How can the GFP Reporter Cell Line - MM1.R be utilized to study the bone marrow microenvironment's influence on multiple myeloma progression?

What advantages does the GFP Reporter Cell Line - MM1.R offer for evaluating the efficacy of CAR-T cell therapy against multiple myeloma in preclinical models?

Can the GFP Reporter Cell Line - MM1.R be employed to investigate the mechanisms of drug resistance in multiple myeloma?

How might researchers use the GFP Reporter Cell Line - MM1.R to explore the role of specific genes in multiple myeloma cell migration and invasion?

In what ways could the GFP Reporter Cell Line - MM1.R facilitate the development and validation of imaging agents for the early detection of multiple myeloma?

Real-Time Monitoring

Quantitative Analysis

Non-Invasive Assay

Visual Gene Expression

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