Cat.No. : CSC-RR0319 Host Cell: 4T1.2
| Cat. No. | CSC-RR0319 |
| Description | 4T1.2-GFP cell line is clonally derived from 4T1.2 cell line which was transduced by a lentiviral vector containing GFP and puromycin resistance gene. GFP is expressed as free cytoplasmatic proteins in these 4T1.2-GFP stable cells. It is a ready-to-use in vitro model for cell-based assay applications. |
| Gene | GFP |
| Host Cell | 4T1.2 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Source | 4T1.2 |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Immunotherapeutic studies of human HER2+ cancer are constrained by the current mouse models, such as transgenic MMTV-erbb2 and MMTV-neu. Researchers used the GFP Reporter Cell Line-4T1.2 cell line to evaluate the impact of human HER2-targeted immunotherapy strategies on the immune system. First, a mouse syngeneic breast cancer model expressing a truncated form of human HER2, HER2T, was generated and its effectiveness was verified. Then, the oncolytic virus VSVΔ51 was combined with the antibody-drug conjugate T-DM1 to treat tumor-bearing mice. Efficacy was assessed by tumor control, survival, and immunoassays. The results showed that combined treatment of VSVΔ51 and T-DM1 significantly improved the therapeutic effect and activated extensive immune memory. Immunoassays revealed activation of CD4+ T cells, B cells, NK cells, and dendritic cells and production of tumor-reactive IgG.
Figure 1. The researchers found that 4T1.2-HER2T cells, treated with T-DM1, enhance sensitivity to VSVΔ51 infection, as evidenced by viral titration and GFP foci quantification following infection at varying MOIs. (Taha Z, et al., 2023)
A: Our GFP Reporter Cell Line-4T1.2 has undergone strict screening and verification to ensure the stable expression of the GFP gene. Through methods such as fluorescence microscopy and flow cytometry, we verified the expression level of GFP and performed repeated tests at different time points and in different batches. The results show that the cells can maintain stable GFP expression under appropriate culture conditions and are suitable for various experimental applications.
A: As a mouse breast cancer cell line, the background of the 4T1.2 cell line may affect cell migration and invasion, but it will not significantly affect the expression of GFP. We recommend that customers pay attention to the special growth requirements of the 4T1.2 cell line during use, such as culture medium components and culture conditions. Detailed culture guidelines will help customers maintain their cells in optimal condition.
A: Yes, our GFP Reporter Cell Line-4T1.2 has undergone rigorous contaminant testing, including detection of common contaminants such as mycoplasma and bacteria. We use methods such as PCR and cell culture testing to ensure the purity of our cell lines. We have strict quality control procedures to ensure that each batch of cell lines meets high standards of purity.
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The performance of GFP Reporter Cell Line-4T1.2 is indeed very stable. During the culture process, we observed consistent cell growth and stable gene expression. Especially the application of these cells in bone metastasis research is really helpful.
The cells' primary tumor characteristics are similar to 4T1, and they also have good bone metastasis capabilities. The cells grew uniformly during the experiment, and there was no instability in gene expression. The expression of GFP gene allows us to clearly observe the tumor spread process, which is very suitable for in-depth cancer research.
Our laboratory has recently used GFP Reporter Cell Line-4T1.2. These cells have stable growth and consistent gene expression. As a platform to study gene regulation and drug efficacy in breast cancer, these cell lines are particularly prone to bone metastasis. The introduction of the GFP reporter gene simplifies our research process and allows us to observe the biological behavior of cells more intuitively. This cell line is indeed a very valuable tool and is recommended to all teams working in cancer research.
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