Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : AD00323Z
Storage : -80℃ Shipping : Frozen on dry ice
Titer: Size:
| Cat. No. | AD00323Z |
| Description | mMKOS stands for 4 mouse stem cell factors which are c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2 ((BC006728 NM_010637 NM_013633 NM_011443). GFP-mMKOS adenovirus Type5 (dE1/E3) uses 2A "self-cleaving" peptides, which support efficient polycistronic expression from a single promoter to reduce the number of viruses necessary to reprogram somatic cells. GFP-mMKOS adenovirus Type5 (dE1/E3) also has a separate expression cassette to express eGFP. Mouse KOSM adenovirus can be used to generate induced pluripotent stem (iPS) cell from mouse and human adult fibroblasts. |
| Product Type | Adenoviral particle |
| Gene | GFP |
| Insert | c-Myc-F2A-Klf4-T2A-Oct4-E2A-Sox2, GFP co-expression |
| Titer | Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc. |
| Size | Varies lot by lot, for example, 100 ul, 500 ul, 1 mL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Summary | Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots. |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance. |
| Sterility | Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination. |
| Ad5 E1 Detection | All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination. |
| RCA Assays | Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources. |
| PFU Titering | All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells. |
| Target Gene | GFP |
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The GFP tag in this adenovirus made it incredibly easy to monitor reprogrammed cells. The fluorescence was bright, and the virus maintained high functionality. Perfect for live-cell imaging studies!
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