GFP Reporter Cell Line - TE-10

Name: GFP Reporter Cell Line - TE-10
SKU: CSC-RR0535
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0535

Host Cell : TE-10 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0535
Description	This cell line is engineered to stably overexpress GFP reporter gene. TE-10 is a human esophageal squamous cancer cell line. This cell line is a useful tool for fluorescent tracking of TE-10 cells.
Target Gene	GFP
Host Cell	TE-10
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0535-GFP Reporter Cell Line - TE-10-Datasheet

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Background

Applications

Q & A

Customer Reviews

The human esophagus epithelial-derived cancer cell line known as TE-10 cells was first obtained in 1975 from a patient who had advanced esophageal cancer and was 58 years of age. These cells are a kind of esophageal squamous cell carcinoma that is well differentiated and resembles epithelial tissue. Since then, a lot of work has been done utilizing the TE-10 cell line to study the causes, symptoms, and treatments of esophageal cancer. Green fluorescent protein (GFP) reporter gene expression is created in the GFP reporter cell line TE-10, which is a useful tool for researching gene expression and biological processes. This cell line is derived from esophageal squamous cell carcinoma in humans, a cancer that is known for its high death rates and aggressive behavior.

1. Esophageal cancer research: The TE-10 cell line is derived from esophageal squamous cell carcinoma in patients. Through GFP reporter gene expression, it can be used to study the pathogenic mechanism, symptoms and treatment methods of esophageal cancer.

2. Gene expression tracking: The GFP reporter gene system in TE-10 cells provides scientists with an effective tool to study gene expression and biological processes at the cellular level.

3. Biological process observation: Using the fluorescent properties of GFP, the dynamics of gene expression inside cells can be observed in real time, and the effects of different treatments on cell behavior can be evaluated.

4. Clinical application model: Because the TE-10 cell line is derived from highly lethal and invasive esophageal squamous cell carcinoma, it provides an important clinical research model for new drug development and treatment strategies.

Customer Q&As

How stable is the expression of the GFP reporter gene?

A: The GFP gene in GFP Reporter Cell Line - TE-10 is integrated into the cell genome through stable transfection technology. The expression level has been verified through multiple generations and remains stable. This stability is very important for long-term experiments and repeated experiments to ensure the consistency and reliability of the results.

How well does this cell line image under a fluorescence microscope?

A: The GFP gene of GFP Reporter Cell Line - TE-10 encodes green fluorescent protein and is suitable for fluorescence microscopy imaging experiments. GFP has high brightness and low photobleaching rate, and can clearly show the structure and dynamic changes inside cells under a fluorescence microscope. This makes this cell line excellent in cell biology studies, especially in studies of cell dynamics, protein localization and interactions, providing high-quality images and reliable data.

How effective is this cell line in studying gene expression regulation?

A: GFP Reporter Cell Line - TE-10 can be used to study gene expression regulatory mechanisms. By detecting changes in GFP expression, the impact of specific regulatory factors or drugs on target genes can be assessed. This cell line is widely used in gene promoter research, transcription factor functional analysis, and drug action mechanism research. Its high sensitivity and stability make experimental data highly credible and can accurately reflect the true situation of gene expression regulation.

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Customer Reviews

Express clearly

The expression of the GFP reporter gene in TE-10 cells is very clear, and the effect is remarkable when observing gene expression and biological processes. In the experiment, the cells grew consistently and the results were highly reproducible.

Germany

2021-11-29

High stability

The cells are very stable during the culture process, and the fluorescence signal of GFP is very strong, making it very intuitive to observe gene expression. The TE-10 cell line is derived from esophageal cancer, has a high degree of tumor differentiation, and the experimental data is very reliable. Fast logistics and high cell activity.

Germany

2020-04-02

Fast logistics

TE-10 GFP reporter cell line arrived quickly, and tightly packaged, and the cells were in good condition. GFP gene expression is very obvious in cells and is suitable for studying gene expression and biological processes. The TE-10 cell line is derived from human esophageal squamous cell carcinoma and exhibits consistent growth and stable gene expression in experiments. Research on the causes and treatments of esophageal cancer is of great help. The logistics service is excellent and the products are of high quality. It is a very satisfying shopping experience.

United States

2024-03-12

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GFP Reporter Cell Line - TE-10

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Applications

Q & A

Customer Reviews

How stable is the expression of the GFP reporter gene?

How well does this cell line image under a fluorescence microscope?

How effective is this cell line in studying gene expression regulation?

Express clearly

High stability

Fast logistics

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