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Cat.No. : CSC-RR0515 Host Cell: MCF10DCIS.com
| Cat. No. | CSC-RR0515 |
| Description | This cell line is engineered to stably overexpress EGFP reporter gene. MCF10DCIS.com cell line is a human breast cancer cell line derived from a xenograft originating from MCF-10AT cells that were injected into severe combined immune-deficient mice. This cell line is a useful tool for fluorescent tracking of MCF10DCIS.com cells. |
| Gene | GFP |
| Host Cell | MCF10DCIS.com |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Breast tumors are frequently identified through manual palpation due to their increased stiffness compared to adjacent normal tissue. Researchers demonstrate that GFP Reporter Cell Line - MCF10DCIS.com enables visualization of cellular responses to matrix stiffness. It elucidates how mechanical cues induce transcriptional changes driving tumour progression, particularly by revealing the TWIST1-G3BP2 mechano transduction pathway underlying epithelial-mesenchymal transition, invasion, and metastasis.
Figure 1. Tumor invasion in vivo was studied using GFP Reporter Cell Line - MCF10DCIS.com. Increased invasion was observed in G3BP2-depleted tumors, as evidenced by enhanced local and regional invasion, as well as lung metastasis, in 3D culture and xenograft models. (Wei SC, et al., 2015)
A: The GFP Reporter Cell Line in MCF10DCIS.com cells provides a valuable tool for investigating breast cancer progression and metastasis. MCF10DCIS.com cells are derived from human breast epithelial cells and exhibit characteristics of ductal carcinoma in situ (DCIS), making them a relevant model for studying early-stage breast cancer. The GFP reporter enables visualization and tracking of cancer cells, facilitating studies on tumor growth, invasion, and metastasis.
A: The fidelity and stability of GFP expression in the MCF10DCIS.com reporter cell line are maintained through rigorous quality control measures. Stable transfection techniques are employed to introduce the GFP reporter gene into MCF10DCIS.com cells, followed by clonal selection to isolate cell lines with stable and consistent GFP expression levels. Regular validation and monitoring of GFP fluorescence ensure reliable data generation in experiments.
A: Yes, the GFP Reporter Cell Line in MCF10DCIS.com cells is suitable for studying epithelial-mesenchymal transition (EMT) and cancer stem cell properties in breast cancer. The GFP fluorescence allows for visualization and tracking of cancer cells undergoing EMT or exhibiting stem cell-like characteristics, providing insights into tumor heterogeneity, metastasis, and therapy resistance.
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The GFP Reporter Cell Line consistently delivers bright signals, ensuring reproducible results and accurate interpretation of experimental outcomes. From investigating oncogenic pathways to screening drug candidates, this cell line serves as a trusted tool for elucidating breast cancer biology and developing targeted therapies.
United States
02/18/2021
The GFP Reporter Cell Line in MCF10DCIS.com cells offers sensitive detection of gene expression and cellular processes, providing valuable data for research in breast cancer biology.
United States
07/13/2022
With GFP fluorescence, this cell line enables precise monitoring of tumor progression, metastasis, and response to therapy, advancing our understanding of breast cancer pathogenesis. Its reliable GFP expression simplifies experimental workflows, facilitating efficient data collection and analysis in breast cancer research.
French
11/13/2023
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