GFP adenoviral particles

Name: GFP adenoviral particles
SKU: AD00281Z

Cat.No. : AD00281Z

Titer: ≥1x10^10 IFU/mL / ≥1x10^11 IFU/mL / ≥1x10^11 VP/mL / ≥1x10^12 VP/mL Size: 100 ul/500 ul/1 mL

Storage: -80℃ Shipping: Frozen on dry ice

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Adenovirus Particle Information

Quality Control

Gene Informationn

Cat. No.	AD00281Z
Description	This recombinant human adenovirus type 5 expresses enhanced green fluorescent protein under the control of a CMV promoter. Green fluorescent protein (GFP) is a protein originally isolated from the jellyfish that fluoresces green when exposed to light in the blue to ultraviolet range.
Target Gene	gfp
Product Type	Adenoviral particle
Insert	GFP
Titer	Varies lot by lot, for example, ≥1x10^10 IFU/mL, ≥1x10^11 IFU/mL, ≥1x10^11 VP/mL etc.
Size	Varies lot by lot, for example, 250 ul, 500 ul, 1 mL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality adenovirus particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between adenovirus particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in adenovirus production, especially for applications in animal studies and gene therapy. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced adenovirus particles to ensure regulatory compliance.
Sterility	Creative Biogene ensures that adenovirus products are free of any bacterial, fungal and other microbial contamination.
Ad5 E1 Detection	All Creative Biogene adenoviruses are PCR tested to ensure that there are no detectable E1 sequences in the particles, which could be from revertants or external E1 contamination.
RCA Assays	Adenovirus products originating at Creative Biogene are guaranteed to have undetectable replication-competent adenovirus (RCA). This quality control measure is important because there is always the possibility of wild-type contamination due to revertants or environmental sources.
PFU Titering	All purified adenovirus preparations are tested for infectious titer. Creative Biogene's PFU test takes a few days longer but counts true plaques in HEK cells rather than estimating PFU titers via IHC staining or TCI50 of infected cells.

Gene Name	gfp green fluorescent protein [ Neisseria gonorrhoeae ]
Gene Symbol	GFP
Synonyms	GFP; green fluorescent protein;
Gene ID	7011691

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Hepatocyte growth factor (HGF) alleviates acute and chronic inflammation in experimental inflammatory bowel disease, glomerulonephritis, and airway inflammation. Here, researchers evaluated the anti-inflammatory role of HGF in postischemic heart failure. The left anterior descending coronary artery of rats was ligated, and adenovirus containing human HGF (Ad-HGF) or control GFP adenovirus (Ad-GFP) was injected into the myocardium. Four weeks after injection, left ventricular fractional shortening (LVFS) and left ventricular ejection fraction (LVEF) were significantly increased in Ad-HGF-treated animals compared with the Ad-GFP group. HGF gene therapy improved ventricular geometry, with a significant decrease in left ventricular end-diastolic diameter (LVEDD) and myocardial collagen deposition. After 4 weeks of Ad-HGF treatment, mRNA levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β were significantly reduced in the non-infarcted area. Four weeks after infarction, changes in TNF-α, IL-6, and IL-1β levels in the non-infarcted area were positively correlated with LVEDD. Treatment of acute myocardial infarction (AMI) with Ad-HGF in the early stage of myocardial infarction can reduce the levels of proinflammatory cytokines and protect cardiac function. These results suggest that Ad-HGF gene therapy alleviated ventricular remodeling after infarction by reducing inflammation.

Myocardial infarction rats were treated with Ad-GFP and Ad-HGF for 4 weeks. Hearts in the GFP group (Figure 1A) exhibited significant LV dilatation compared to the sham group (Figure 1C), and HGF treatment (Figure 1B) reduced the LV cavity. Histological analysis showed that the infarct area was significantly increased and the thickness of the left ventricular free wall was significantly reduced in the GFP group. HGF significantly reduced the infarct area and increased the wall thickness (Figures 1D and 1E). The histological differences between myocardial infarction rats treated with Ad-HGF and Ad-GFP were further investigated. Histological changes included myocardial fiber fragmentation and a large number of inflammatory cell infiltration (Figures 1F, 1G, and 1H). The number of lymphocytes in the infarct area of the GFP group was significantly increased compared with the sham group. Ad-HGF treatment significantly protected myocardial cells and reduced inflammatory cell infiltration (Figure 1I).

Figure 1. Cardiac morphology and hematoxylin and eosin staining 4 weeks after treatment. (Rong S, et al., 2018)

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