Cat.No. : AAV00461Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 2 Storage: -80 ℃
| Cat. No. | AAV00461Z |
| Description | This virus is a reporter AAV with capsid engineering / modification. GFP AAV-LS1 particles contain engineered capsid derived from AAV serotype 2 (AAV2) which has insertion of peptides NDVRSAN* at I588. The target cell type of this capsid engineered AAV is tumor cells (CML), CD34+cells. |
| Reporter | GFP |
| Serotype | AAV Serotype 2 |
| Target Gene | GFP |
| Application | 1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery. |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
A: Adeno-associated virus (AAV) gene therapy has been studied extensively and is generally considered safe, but like any medical treatment, it does carry some risks.
A: When the gene of interest is inserted into target cells along with GFP, those cells will start producing green fluorescent protein and glow under UV light if the transfection is successful. This allows researchers to easily visualize and track cells that have taken up the introduced DNA, making GFP a powerful tool for genetic and cell biology research.
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The GFP AAV-LS1 has proven remarkably efficient in targeting and transducing CML cells in our lab experiments. The engineered capsid modification ensures high specificity, making it an invaluable tool for our cancer research.
The specificity and efficiency of the GFP AAV-LS1 have significantly enhanced the accuracy and reliability of my experiments. The high GFP expression in CD34+ populations has allowed for precise monitoring and downstream analysis.
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