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GFP Reporter Cell Line - RL

GFP Reporter Cell Line - RL

Cat.No. :  CSC-RR0590 Host Cell:  RL

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Cat. No. CSC-RR0590
Description This cell line is engineered to stably express GFP reporter gene in RL cells.
Gene GFP
Host Cell RL
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Reporter Type Fluorescent protein
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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Customer Reviews

The OCI-AML-3 cell line is a human acute myeloid leukemia (AML) cell line derived from the peripheral blood of a 57-year-old Caucasian male with AML FAB M4. This cell line is widely used in leukemia research due to its well-characterized genetic profile, including mutations in the NPM1 and DNMT3A genes, which are critical for understanding the pathogenesis and therapeutic resistance of AML. The GFP/Luc Reporter OCI-AML-3 cell line is a modified version of the original OCI-AML-3, engineered to stably express both green fluorescent protein (GFP) and luciferase. The GFP tag allows for easy visualization and tracking of cells using fluorescence microscopy or flow cytometry, while the luciferase reporter allows for non-invasive bioluminescence imaging in living organisms. This dual reporter system is particularly valuable for in vivo studies and preclinical testing of anti-leukemia drugs, providing real-time, quantifiable readouts of cell viability, proliferation, and therapeutic response.
GFP Reporter Cell Line - RL is a powerful tool in modern biomedical research. This cell line has been engineered to stably express the Green Fluorescent Protein (GFP) reporter gene in RL cells. Here are some of its key applications: Gene Expression Analysis: The GFP Reporter Cell Line - RL allows researchers to visualize and quantify gene expression in real time. Since GFP fluorescence can be easily detected using fluorescence microscopy or flow cytometry, the GFP Reporter Cell Line - RL is very useful for studying gene regulation and promoter activity. Drug Screening: This cell line can be used in high-throughput screening assays to test the effects of various compounds on gene expression. Using GFP as a readout, researchers can quickly identify drugs that modulate the activity of specific genes or pathways. Signal Transduction Studies: Stable expression of GFP facilitates the study of intracellular signaling pathways. Changes in fluorescence intensity can indicate alterations in the signal transduction process, allowing the study of cellular responses to different stimuli. Cell Tracking: GFP Reporter Cell Line - RL can be easily tracked in vitro and in vivo, providing important insights into cell migration, proliferation, and differentiation. This is particularly useful in cancer research, where understanding tumor cell behavior is critical to developing new therapies. Protein-Protein Interactions: The GFP tag can be used in combination with other fluorescent proteins to study protein-protein interactions through techniques such as FRET (Fluorescence Resonance Energy Transfer). This helps to elucidate the complex network of protein interactions within cells.
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Customer Reviews
High sensitivity and specificity

We have tested several reporter cell lines and GFP Reporter Cell Line - RL stands out for its high sensitivity and specificity. Highly recommended for any lab doing gene expression studies.

Germany

09/21/2020

Cost-effective

For labs on a budget, the GFP Reporter Cell Line - RL offers incredible value. It delivers high performance without breaking the bank, and it’s scalable for larger studies.

French

08/28/2020

Fantastic service!

The customer support for the GFP Reporter Cell Line - RL is top-notch. Not only is the product itself excellent, but the team behind it is incredibly helpful and responsive. They answered all our queries promptly and provided valuable troubleshooting tips.

French

07/27/2022

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