GFP Reporter Cell Line - CHO-S

Name: GFP Reporter Cell Line - CHO-S
SKU: CSC-RR0466

Cat.No. : CSC-RR0466 Host Cell: CHO-S

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0466
Description	CHO-S-GFP is a cell line that is engineered to stably express GFP. This cell line is an ideal tool to support research in fluorescent tracing of CHO-S cells.
Gene	GFP
Host Cell	CHO-S
Host Cell Species	Cricetulus griseus (Chinese hamster)
Stability	Validated for at least 10 passages
Reporter Type	Fluorescent protein
Application	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Shipping	Dry ice
Storage	Liquid nitrogen

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Background

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Advantage of GFP is its non-toxic nature. The protein is inert and does not interfere with cellular processes, making it safe to use in experiments involving living organisms, including humans. This feature is particularly important in the field of medicine, where GFP is used to study disease mechanisms and develop new diagnostic and therapeutic strategies.

GFP also has a high expression level in various cell types and organisms, making it a versatile tool for genetic engineering and expression studies. Its strong fluorescence allows for easy detection and quantification, reducing the need for extensive sample processing and increasing the efficiency of experiments.

CHO-S Cell Line is a Chinese Hamster Ovary (CHO) cell line modified for high productivity in the production of recombinant proteins. It is widely used in the biotechnology and pharmaceutical industries for the production of therapeutic drugs.CHO-S cells are known for their robust growth, high transgene expression, and low background noise for protein production. They are also capable of secreting a wide range of proteins, including monoclonal antibodies. Advantages of using CHO-S cells include reliable and stable performance, ease of culture, and optimised culture conditions. In summary, the CHO-S cell line is a valuable tool for the efficient production of important biopharmaceuticals.

The researchers demonstrated that sortilin facilitates the uptake of lipidated apoE, crucial for converting polyunsaturated fatty acids (PUFA) into neuromodulators that promote anti-inflammatory gene expression in the brain. This pathway, essential for neuroprotection, functions effectively with the apoE3 variant but is compromised by apoE4, the primary genetic risk factor for Alzheimer's disease (AD). Through unbiased proteome screens and mouse model analyses, the researchers revealed that sortilin interacts with fatty acid-binding protein 7 (FABP7), an intracellular carrier of PUFA in the brain. In the presence of apoE3, sortilin enhances the functional expression of FABP7, thereby promoting lipid-dependent gene transcription. Conversely, apoE4 binding inhibits sortilin-mediated processes, leading to FABP7 degradation and impaired lipid signaling. These findings underscore the critical role of sortilin in maintaining neuronal lipid homeostasis and provide insights into the disrupted lipid metabolism observed in AD patients expressing apoE4.

Figure 1 demonstrates the role of sortilin by stabilizing FABP7 protein levels in CHO cells and CHO-S cells expressing sortilin. (doi: 10.1242/jcs.258894) Figure 1. The researchers transiently transfected FABP7 and GFP-expressing constructs with either parental CHO cells or CHO-S cells stably expressing sortilin, and analyzed FABP7 levels by Western blot and densitometric scanning, using GFP and tubulin as controls. (Asaro A, et al., 2021)

Customer Q&As

What is GFP Reporter CHO-S Cell Line?

A: GFP Reporter CHO-S Cell Line is a Chinese hamster ovary (CHO) cell line that has been genetically modified to express green fluorescent protein (GFP). This cell line is widely used in molecular and cell biology research for monitoring gene expression and protein localization in real-time.

How is the GFP Reporter CHO-S Cell Line useful in research?

A: The GFP Reporter CHO-S Cell Line is particularly valuable for researchers who need to study the regulation and dynamics of gene expression. By fusing GFP to their target protein or using GFP as a reporter, researchers can easily visualize and track the expression and localization of their interest protein using fluorescence microscopy.

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