scAAV DJ/8-CAG-GFP

Name: scAAV DJ/8-CAG-GFP
SKU: AAV00410Z
Rating: 4.0 (1 reviews)

Cat.No. : AAV00410Z

Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml

Serotype: AAV serotype DJ/8 Storage: -80 ℃

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AAV Particle Information

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Cat. No.	AAV00410Z
Description	Premade self-complementary AAV particles in serotype DJ/8 (scAAV DJ/8) express GFP reporter gene from the CAG promoter.
Serotype	AAV serotype DJ/8
Target Gene	GFP
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Customer Reviews

High purity and titer

The high purity and titer of the scAAV DJ/8-CAG-GFP preps from Creative Biogene have consistently provided us with strong transgene expression and minimal toxicity, improving the overall quality of our experiments.

Germany

05/25/2023

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scAAV DJ/8-CAG-GFP

AAV Particle Information

Quality Control

Publications

Q & A

Customer Reviews

High purity and titer

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