Cat.No. : AAV00320Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV serotype Retrograde Storage: -80 ℃
| Cat. No. | AAV00320Z |
| Description | Prepackaged AAV particles in serotype retrograde containing EGFP reporter gene under the control of CAG promoter. |
| Serotype | AAV serotype Retrograde |
| Target Gene | GFP |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
The mitochondrial permeability transition pore is a nonspecific transmembrane channel, and inhibition of mitochondrial permeability transition pore opening has been shown to reduce mitochondrial swelling, calcium overload, and axonal degeneration. In this study, an in vivo ICH mouse model was established by injecting autologous blood and oxygenated hemoglobin into the striatum of Thy1-YFP mice, in which pyramidal neurons and axons expressed yellow fluorescent protein. The researchers found that early axonal degeneration in ICH was dependent on mitochondrial swelling and mitochondrial permeability transition pore opening caused by cyclophilin D (CypD) activation. Both cyclosporin A inhibition and short hairpin RNA interference of cyclosporin D reduced mitochondrial permeability transition pore opening and mitochondrial damage. In addition, inhibition of cycloserine proteinase D and mitochondrial permeability transition pore opening protected the integrity of the corticospinal tract and alleviated motor dysfunction caused by ICH. These findings suggest that cycloserine proteinase D is a key mediator of axonal degeneration after ICH; inhibition of cycloserine proteinase D expression protects mitochondrial structure and function, further alleviating corticospinal tract damage and motor dysfunction after intracerebral hemorrhage.
Motor outcome after stroke depends on the integrity of the corticospinal tract (CST). To assess the integrity of the CST and CST-associated fine motor function after Intracerebral hemorrhage (ICH), viral retrograde tracking (AAV2/Retro-CAG-GFP) from the lumbar spinal cord and fine motor behavioral tests were performed. The number of retrogradely labeled GFP-positive corticospinal neurons in the ICHCypD–/– and ICHWT + CsA (Figure 1A and B) groups was greater than that in the ICHWT group. The slip rates in the beam walking and irregular ladder walking tests were decreased in the ICHCypD–/– and ICHWT + CsA groups at 3 days after ICH compared with those in the ICHWT group (Figure 1C and D). These results suggest that inhibition of CypD promotes the integrity of the CST and facilitates functional recovery after ICH.
Figure 1. CypD deficiency and CsA treatment protect the CST and alleviate motor dysfunction after ICH. (Yang Y, et al., 2023)
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