Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR00831
Host Cell : SiHa Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR00831 |
| Description | This cell line is engineered to stably express GFP reporter gene in SIHA cells. It is an ideal cell line model in fluorescent tracking of SIHA cells. |
| Product Type | Stable cell line constitutively expressing GFP reporter gene |
| Target Gene | GFP |
| Host Cell | SiHa |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1) in vitro cell tracking by fluorescent signal 2) monitor in vivo tumor growth using the fluorescence signal 3) anticancer drug development |
| Size | One vial of frozen cells, typically >1x10^6cells/vial |
| Stability | This cell line is stable at least 10 passages. |
| Quality Control |
1) cell line imaging under fluorescent microscopy 2) mycoplasma detection |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Target Gene | GFP |
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We purchased the SIHA GFP reporter line for our HPV-related cervical cancer studies, and it has been outstanding. The GFP expression is bright, uniform, and stable — we consistently see >95% GFP-positive cells by flow cytometry even after multiple passages.
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