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POU5F1-GFP Reporter Cell Line-H9 hESC

POU5F1-GFP Reporter Cell Line-H9 hESC

Cat.No. :  CSC-RR0323 Host Cell:  H9 hESC

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Cell Line Information

Cell Culture Information

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Cat. No. CSC-RR0323
Description This cell line expresses POU5F1-GFP fusion protein as a reporter of POU5F1 activity when it is maintained as undifferentiated embryonic stem cell. It is a good ready-to-use cell model for studying human embryonic stem cell self-renewal and differentiation.
Gene GFP
Host Cell H9 hESC
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Application

1. Gene expression studies

2. Protein localization

3. Drug screening and toxicology

4. Live cell imaging

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Source H9 hESC
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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