Cat.No. : CSC-RR0581 Host Cell: KU-812E
| Cat. No. | CSC-RR0581 |
| Description | This cell line is engineered to stably express GFP reporter gene in KU-812E cells. |
| Gene | GFP |
| Host Cell | KU-812E |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
CML is primarily treated with TKIs like Imatinib, which inhibit the Bcr-Abl protein, improving patient survival. Researchers explored alternative strategies for treating CML due to current therapy limitations, notably TKIs. They investigated SMO inhibition within the Hedgehog signaling pathway. Using Cell Line KU-812, they tested SMO-inhibiting compounds, notably MRT92, observing reductions in Gli1 protein levels, increased Gli1 and SMO RNA levels, decreased cell proliferation, and induced apoptosis/autophagy. Additionally, these compounds modulated miRNAs associated with the Hedgehog pathway, suggesting SMO inhibition as a promising therapeutic target for CML, with potential as pathway antagonists.
Figure 1. SMO RNA expression in KU-812 cells was investigated by the researchers. The effects of compounds MRTX, MRT94, MRT92, MRT83, MRTY, and a control compound on SMO RNA expression after 24h (a) or 72h (b) treatment at 10 μM and after 24h (c) or 72h (d) treatment at 50 μM were assessed. (Chiarenza A, et al., 2016)
If your interest lies in studying changes in transcriptional activity, you can utilize the GFP Reporter Cell Line - KU-812E from Creative Biogene to directly monitor GFP reporter gene expression, obviating the need for RT-qPCR and western blot analyses. This approach streamlines the experimental workflow and enables real-time monitoring of transcriptional activity. Furthermore, employing the GFP Reporter Cell Line reduces cellular manipulations, thereby mitigating the risk of cell damage and enhancing the repeatability and reliability of experiments.
A: The KU-812E GFP Reporter Cell Line maintains reliable GFP expression levels through optimized transfection protocols and careful clonal selection, resulting in consistent and stable GFP fluorescence suitable for various research applications.
A: The KU-812E GFP Reporter Cell Line provides a valuable model for investigating myeloid leukemia biology, including cell differentiation, drug response, and disease progression. GFP expression allows for easy tracking and monitoring of myeloid leukemia cells in vitro and in vivo.
A: The KU-812E GFP Reporter Cell Line can be utilized in drug screening assays to evaluate the efficacy of potential therapeutics against myeloid leukemia. GFP fluorescence serves as a reliable readout for assessing drug-induced cytotoxicity or inhibitory effects on leukemia cell proliferation.
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Consistent performance! The GFP Reporter Cell Line consistently provides robust signals, ensuring reproducible results and accurate interpretation of experimental outcomes. From studying tumor microenvironment interactions to evaluating treatment responses, this cell line serves as an invaluable resource for unraveling the complexities of leukemia biology and developing novel therapeutic strategies.
Streamlined experimentation! Its stable GFP expression simplifies experimental workflows, allowing for efficient data collection and analysis in leukemia research.
With GFP fluorescence, this cell line facilitates comprehensive investigation of tumor growth, metastasis, and response to therapy, advancing our understanding of leukemia biology. The GFP Reporter Cell Line in KU-812E cells offers clear and vibrant GFP expression, enabling detailed visualization and analysis of cellular processes in my leukemia research.
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