GFP Adeno-Associated Virus ( AAV-MecB )

Name: GFP Adeno-Associated Virus ( AAV-MecB )
SKU: AAV00428Z
Rating: 4.0 (2 reviews)

Cat.No. : AAV00428Z

Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml

Serotype: AAV Serotype 2 Storage: -80 ℃

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AAV Particle Information

Quality Control

Cat. No.	AAV00428Z
Description	This virus is a reporter AAV with capsid engineering / modification. GFP AAV-MecB particles contain engineered capsid derived from AAV serotype 2 (AAV2) which has insertion of peptides RSNAVVP at I587. The target cell type of this capsid engineered AAV is tumor cells.
Reporter	GFP
Serotype	AAV Serotype 2
Target Gene	GFP
Application	1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery.
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

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Background

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Customer Reviews

Adeno-associated virus serotype 2 (AAV2) is a widely studied vector for gene therapy because it is safe and able to infect both dividing and non-dividing cells. However, natural AAV2 capsids have limitations, such as limited tropism and potential immunogenicity. To overcome these challenges, engineered AAV2 capsids have been developed. These capsid modifications are designed to enhance the properties of AAV2, thereby expanding its clinical applications.

Engineered AAV2 capsids are created through various methods, such as rational design, directed evolution, and recombination. Rational design involves making specific changes to exposed amino acids on the capsid surface to improve its ability to target specific tissues or evade neutralizing antibodies. Directed evolution, on the other hand, requires the generation of a large library of capsid variants through random mutagenesis, followed by repeated selection for desired properties. Recombination combines elements from different serotypes to produce new chimeric capsids with enhanced properties.

Customer Q&As

What is GFP?

A: Green fluorescent protein (GFP) is a protein that glows with a bright green fluorescence under ultraviolet light.

Is AAV the smallest DNA virus?

A: AAV is the smallest DNA virus with an average size of 20 nm.

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Customer Reviews

Precise and efficient transduction of tumor cells

The capsid engineering with the RSNAVVP peptide insertion at I587 significantly enhanced its selectivity, which allowed us to observe precise and efficient tumor cell transduction in our experimental models.

Canada

07/09/2022

High-Quality Fluorescence Imaging

The clarity and intensity of the GFP signal made it easier to track and analyze tumor cell behavior in real-time, enhancing the accuracy of our research outcomes.

French

07/13/2021

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GFP Adeno-Associated Virus ( AAV-MecB )

AAV Particle Information

Quality Control

Background

Publications

Q & A

Customer Reviews

What is GFP?

Is AAV the smallest DNA virus?

Precise and efficient transduction of tumor cells

High-Quality Fluorescence Imaging

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