GFP Adeno-Associated Virus ( AAV-DIIRA )

Targeted gene transfer has been a major area of ​​research in molecular medicine over the past decade. For AAV vectors, the use of serotypes with unique transduction profiles and the introduction of targeting peptides in the capsid (primarily AAV2) as well as DNA shuffling of different serotypes have been explored to achieve targeted gene delivery. For several reasons, the capsid region surrounding amino acid R588 (VP numbering) is most commonly used to incorporate peptide ligands into AAV2. Such peptide insertions are compatible with capsid assembly. In addition, the targeting peptide occurs 60 times near the top of the protruding capsid domain within the triple spike region, promoting interactions with potential cell surface structures.

Green fluorescent protein adeno-associated virus (GFP AAV-DIIRA) is an advanced tool in the field of gene delivery that is unique in that it is derived from an engineered capsid of adeno-associated virus serotype 2 (AAV2). This viral vector has been carefully enhanced by capsid modification at position I588, into which the DIIRA peptide is inserted. The engineered GFP AAV-DIIRA particles show a pronounced affinity for endothelial cells that form the lining of blood vessels. This specificity makes GFP AAV-DIIRA particularly valuable in studying diseases and conditions involving the vascular system, such as atherosclerosis, hypertension, and other cardiovascular diseases.