Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RR0353
Host Cell : SK-MEL-30 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RR0353 |
| Description | SK-MEL-30 - GFP cell line is constructed by transfection of GFP encoding vector into SK-MEL-30 cells, followed by stable cell selection. GFP is stably expressed as free cytoplasmatic protein in this cell line. |
| Background | SK-MEL-30 melanoma is a human tumor cell line used for research as a model. |
| Target Gene | GFP |
| Host Cell | SK-MEL-30 |
| Host Cell Species | Homo sapiens (Human) |
| Source | SK-MEL-30 |
| Reporter Type | Fluorescent protein |
| Applications |
1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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The GFP SK-MEL-30 cells from Creative Biogene are very healthy and easy to maintain. The GFP signal intensity is vivid and doesn't fade, which has greatly improved the quality of our confocal microscopy images. A very reliable product for melanoma modeling.
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