CAG-GFP Adeno-associated virus(AAV Serotype 6)

AAV is a small, non-enveloped virus with a single-stranded DNA genome. Among the various serotypes, AAV6 stands out for its efficient transduction ability and broad tissue tropism. This serotype shows a natural tendency to target respiratory, muscle, and liver tissues, promoting its application in the treatment of diseases related to these organs. AAV6 is able to transduce primary human cells, including airway epithelial cells, hematopoietic stem cells, and cardiac and skeletal muscle cells. This makes it particularly suitable for the treatment of genetic diseases affecting these cell types. The combination of AAV6 vectors with GFP under the control of the CAG promoter provides a powerful tool for basic science and therapeutic approaches to study gene function and expression dynamics in organisms in detail. The CAG promoter, a combination of the Cytomegalovirus (CMV) early enhancer element and chicken beta-actin promoter, is commonly used for robust and ubiquitous expression in different cell types. It drives the expression of green fluorescent protein (GFP), a widely used reporter protein that allows researchers to visualize and quantify the expression of target genes.