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Cat.No. : CSC-RR0521 Host Cell: MC38
| Cat. No. | CSC-RR0521 |
| Description | This cell line is engineered to stably overexpress GFP reporter gene. MC38 is a murine colon carcinoma cell line, which has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of MC38 cells. |
| Gene | GFP |
| Host Cell | MC38 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
Checkpoint blockade immunotherapies that target T-cell co-inhibitory signaling pathways are transforming cancer treatment. The researchers addressed key challenges of current immune checkpoint inhibitors, namely low response rates and immune-related adverse effects (irAEs), by combining immunogenic chemotherapy with a locally expressed PD-L1 trap fusion protein. They found that oxaliplatin (OxP) enhances the efficacy of anti-PD-L1 monoclonal antibody therapy in murine colorectal cancer. By using a lipid-protamine-DNA nanoparticle to deliver the coding plasmid for the PD-L1 trap, the trap is produced transiently and locally within the tumor microenvironment. This approach, combined with OxP, effectively inhibits tumors and demonstrates improved tolerance compared to the combination of OxP and anti-PD-L1 monoclonal antibodies, as it does not significantly increase Th17 cell accumulation in the spleen. This study suggests that locally expressed PD-L1 traps could offer a promising alternative for cancer therapy, particularly when used alongside OxP-based chemotherapy.
Figure 1. The GFP/Luc Reporter Cell Line - MC38 enables real-time monitoring of tumor growth and treatment response, making it essential for evaluating checkpoint inhibitor therapies in MMR-deficient colorectal cancer models. (Song W, et al., 2018)
A: The GFP Reporter Cell Line - MC38 can be utilized to assess the efficacy of novel immunotherapeutic agents by monitoring changes in GFP expression as a surrogate marker for cell viability and proliferation. Researchers can treat these cells with various concentrations of immunotherapeutic agents and measure GFP fluorescence intensity through flow cytometry or fluorescence microscopy. A decrease in GFP fluorescence compared to control treatments indicates effective agent-induced cytotoxicity or inhibition of cell proliferation. This approach allows for the rapid screening of potential immunotherapeutics targeting colorectal cancer in a high-throughput manner.
A: When utilizing the GFP Reporter Cell Line - MC38 in murine models for in vivo imaging, considerations include choosing the appropriate imaging system (e.g., fluorescence or bioluminescence imaging) that offers sufficient sensitivity and depth penetration to detect GFP expression in vivo. Additionally, optimizing the timing of imaging post-injection to allow for adequate tumor growth and GFP expression, as well as minimizing background fluorescence from the diet or fur pigmentation, is crucial. Employing proper anesthesia protocols to reduce stress and movement during imaging, and using software tools for quantitative analysis of GFP signal intensity, can further enhance the reliability and reproducibility of the results.
A: Yes, the GFP Reporter Cell Line - MC38 can be applied in co-culture systems to study the impact of the tumor microenvironment on colorectal cancer progression. Using these cells in co-culture with immune cells, fibroblasts, or endothelial cells can provide insights into cell-cell interactions, cytokine signaling, and the effects of the microenvironment on tumor growth and metastasis. Methodologies such as real-time live-cell imaging to track GFP-expressing tumor cells, flow cytometry to analyze cell populations, and co-culture assays under hypoxic or normoxic conditions can facilitate this research. Advanced techniques like confocal microscopy can also be used to observe the spatial relationships and interactions between MC38 cells and components of the tumor microenvironment.
A: To ensure stable maintenance of GFP expression in the GFP Reporter Cell Line - MC38 during long-term culture, strategies include using a consistent and optimized cell culture medium that supports the health and viability of these cells. Regularly checking for GFP expression through fluorescence microscopy and performing flow cytometry analysis can help monitor the stability of GFP expression. It's also essential to maintain the cells under selective pressure if the GFP expression is under the control of an antibiotic resistance gene. Additionally, limiting the number of passages and freezing early passage stocks can preserve the integrity and expression levels of GFP in the cell line.
A: The GFP Reporter Cell Line - MC38 facilitates the study of metastatic behavior in colorectal cancer by enabling the visualization and tracking of tumor cells in vivo and in vitro. Experimental designs that could maximize insights include the use of orthotopic implantation of GFP-expressing MC38 cells into relevant mouse models to monitor primary tumor growth and the formation of distant metastases over time using in vivo imaging systems. Additionally, employing invasion and migration assays in vitro can provide mechanistic insights into the processes driving metastasis. Analysis of GFP fluorescence can quantify the extent of cell migration and invasion under various conditions, such as the presence of metastasis-promoting or inhibiting agents. These approaches allow for the detailed study of the factors influencing colorectal cancer metastasis and the potential development of targeted therapeutic strategies.
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The GFP Reporter Cell Line - MC38 allows for direct visualization of gene expression in real-time, using the green fluorescent protein (GFP) as a marker. This feature facilitates the study of gene activity and protein localization within cells, providing immediate visual feedback and enabling precise tracking of cellular dynamics without disrupting cellular integrity.
United States
08/29/2020
GFP in the GFP Reporter Cell Line - MC38 is known for its high fluorescence efficiency, ensuring that even low levels of expression are detectable. This high sensitivity is crucial for experiments requiring the detection of subtle changes in gene expression, allowing us to accurately quantify and analyze results.
United States
01/27/2024
One of the key advantages of using GFP in the GFP Reporter Cell Line - MC38 is its non-toxic nature to cells. This allows for prolonged studies involving live cells without affecting cellular viability or functionality. We can perform extended observations and experiments without worrying about adverse effects on the cells under study.
Canada
03/12/2020
The GFP Reporter Cell Line - MC38 is particularly useful for analyzing promoter activity. By linking GFP expression to specific promoters, we can assess the activity of these promoters under various conditions, aiding in the understanding of gene regulation mechanisms and the effects of different stimuli on gene expression.
United States
07/30/2020
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