GFP Reporter Cell Line - SU-DHL-4

The researchers investigated the impact of Raji-derived extracellular vesicles (Evs) on lymphoma cells, with a focus on proliferation, invasion, autophagy, and apoptosis. Methods included Cell Counting Kit-8 (CCK-8) assays, Transwell experiments, microscopy, Western blotting, and flow cytometry. They analyzed miR-106a expression, downregulated it in Raji cells, and isolated resulting Evs (Evs-in-miR-106a). The binding relationship between miR-106a and Beclin1 was validated through RNA pull-down and dual-luciferase reporter gene assays. Overexpression of Beclin1 in SU-DHL-4 and Farage cells was performed, assessing autophagy and apoptosis in SU-DHL-4 cells. Tumorigenicity assays in nude mice validated the effects of Raji-Evs. Results demonstrated that Raji-Evs promoted lymphoma cell proliferation and invasion by upregulating miR-106a, which was reversed upon miR-106a knockdown. Furthermore, Raji-Evs-carried miR-106a targeted Beclin1, inhibiting autophagy and apoptosis, thus facilitating tumor growth in vivo. This study elucidated the mechanism by which Raji-Evs inhibit lymphoma cell autophagy and apoptosis while promoting cell growth via the miR-106a/Beclin1 axis.

Figure 1. Inhibition of autophagy and apoptosis in SU-DHL-4 cells via the miR-106a/Beclin1 axis was observed, employing TEM, GFP-mRFP-LC3, WB, flow cytometry, and RT-qPCR techniques. (Tang J, et al., 2022)

If similar studies are conducted, the utilization of our GFP Reporter Cell Line - SU-DHL-4 GFP reporter gene cell line would streamline the experimental procedures described. Within a unified cellular model, simultaneous monitoring of autophagy, apoptosis, and related protein expression levels can be achieved. This encompasses fluorescence microscopy for autophagic pathway observation, Western blot analysis for protein expression levels, and RT-qPCR for miRNA and mRNA expression analysis. These advancements simplify the assessment of cellular processes, thereby enhancing experimental efficiency and data coherence.