Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Premade Virus Particles
Ready-to-Use | High Titer | Versatile Applications
Premade AAV, adenovirus, lentivirus particles, safe, stable, in stock.
Virus-Like Particles (VLPs)
Stable | Scalable | Customizable
Advanced VLPs for vaccine development (Chikungunya, Dengue, SARS-CoV-2), gene therapy (AAV1 & AAV9), and drug screening (SSTR2, CCR5).
Oligonucleotide Products
Precise | High Yield | Tailored Solutions
Accelerate your research with cost-effective LncRNA qPCR Array Technology.
RNA Interference Products
Targeted | Potent | High Specificity
Human Druggable Genome siRNA Library enables efficient drug target screening.
Recombinant Drug Target Proteins
Authentic | Versatile | Accelerated
Providing functional, high-purity recombinant proteins—including membrane proteins and nanodiscs—to overcome bottlenecks in drug screening and target validation.
Clones
Validated | Reliable | Comprehensive Collection
Ready-to-use clones for streamlined research and development.
Kits
Complete | Convenient | High Sensitivity
Chromogenic LAL Endotoxin Assay Kit ensures precise, FDA-compliant endotoxin quantification for biosafety testing.
Enzymes
Purified | Stable | Efficient
Powerful Tn5 Transposase for DNA insertion and random library construction.
Aptamers
Highly Specific | Robust | Versatile
Aptamers for key proteins like ACVR1A, Akt, EGFR, and VEGFR.
Adjuvants
Enhancing | Synergistic | Effective
Enhance immune responses with high-purity, potent CpG ODNs.
Laboratory Equipment
Innovative | Reliable | High-Precision
Effortlessly streamline DNA extraction with CB™ Magnetic-Nanoparticle Systems.
Stable Cell Line Generation
Reliable | Scalable | Customizable
Fast proposals, regular updates, and detailed reports; strict quality control, and contamination-free cells; knockout results in 4-6 weeks.
Target-based Drug Discovery Service
Innovative | Comprehensive | Efficient
Target identification, validation, and screening for drug discovery and therapeutic development.
Custom Viral Service
Versatile | High-Yield | Safe
Unbeatable pricing, fully customizable viral packaging services (covering 30,000+ human genes, 200+ mammals, 50+ protein tags).
Custom Antibody Service
Precise | Flexible | Efficient
End-to-end antibody development support, from target to validation, enabling clients to rapidly obtain application-ready antibodies.
Antibody-Drug Conjugation Service
Integrated | Controlled | Translational
Comprehensive solutions covering design, development, and validation to ensure conjugated drugs with consistent quality and clinical potential.
Protein Degrader Service
Efficient | High-Precision | Advanced Therapeutics
Harness the power of protein degraders for precise protein degradation, expanding druggable targets and enhancing therapeutic effectiveness for cutting-edge drug discovery.
Nucleotides Service
Accurate | Flexible | High-Quality
Custom synthesis of oligonucleotides, primers, and probes for gene editing, PCR, and RNA studies.
Custom RNA Service
Custom RNA ServicePrecise | Flexible | GMP-ReadyCustom
RNA design, synthesis, and manufacturing—covering mRNA, saRNA, circRNA, and RNAi. Fast turnaround, rigorous QC, and seamless transition from research to GMP production.
Custom Libraries Construction Service
Comprehensive | High-throughput | Accurate
Custom cDNA, genomic, and mutagenesis libraries for drug discovery, screening, and functional genomics.
Gene Editing Services
Precise | Efficient | Targeted
Gene editing solutions for gene editing, knockouts, knock-ins, and customized genetic modifications. Integrated multi-platform solutions for one-stop CRISPR sgRNA library synthesis and gene screening services
Microbe Genome Editing Service
Precise | Scalable | Customizable
Enhance microbial productivity with advanced genome editing using Rec-mediated recombination and CRISPR/Cas9 technologies.
Biosafety Testing Service
Reliable | Comprehensive | Regulated
Complete biosafety testing solutions for gene therapy, viral vectors, and biologics development.
Plant Genetic Modification Service
Advanced | Sustainable | Tailored
Genetic modification for crop improvement, biotechnology, and plant-based research solutions.
Plant-based Protein Production Service
Efficient | Scalable | Customizable
Plant-based protein expression systems for biopharmaceuticals, enzyme production, and research.
Aptamers Service
Innovative | Fast | Cost-Effective
Revolutionizing drug delivery and diagnostic development with next-generation high-throughput aptamer selection and synthesis technologies.
CGT Biosafety Testing
Comprehensive | Accurate | Regulatory-compliant
Internationally certified evaluation system for biologics, gene therapies, nucleic acid drugs, and vaccines.
Pandemic Detection Solutions
Rapid | Precise | Scalable
Balancing accuracy, accessibility, affordability, and rapid detection to safeguard public health and strengthen global response to infectious diseases.
cGMP Cell Line Development
Reliable | Scalable | Industry-leading
Stable expression over 15 generations with rapid cell line development in just 3 months.
Supports adherent and suspension cell lines, offering MCB, WCB, and PCB establishment.
GMP mRNA Production
Efficient | Scalable | Precise
Scalable mRNA production from milligrams to grams, with personalized process design for sequence optimization, cap selection, and nucleotide modifications, all in one service.
GMP Plasmid Production
High Quality | Scalable | Regulatory-compliant
Our plasmid production services span Non-GMP, GMP-Like, and GMP-Grade levels, with specialized options for linearized plasmids.
GMP Viral Vector Manufacturing
Scalable | High Yield | Quality-driven
Advanced platforms for AAV, adenovirus, lentivirus, and retrovirus production, with strict adherence to GMP guidelines and robust quality control.
AI-Driven Gene Editing and Therapy
Innovative | Precision | Transformative
AI-powered one-click design for customized CRISPR gene editing strategy development.
AI-Antibody Engineering Fusion
Next-Generation | Targeted | Efficient
AI and ML algorithms accelerate antibody screening and predict new structures, unlocking unprecedented possibilities in antibody engineering.
AI-Driven Enzyme Engineering
Smart | Efficient | Tailored
High-throughput enzyme activity testing with proprietary datasets and deep learning models for standardized and precise enzyme engineering design.
AI-Enhanced Small Molecule Screening
Predictive | Efficient | Insightful
Leverage AI to uncover hidden high-potential small molecules, prioritize leads intelligently, and reduce costly trial-and-error in early drug discovery.
AI-Driven Protein Degrader Drug Development
Innovative | Targeted | Accelerated
Use AI-guided design to optimize protein degraders, addressing design complexity and enhancing efficacy while shortening development timelines.
Cat.No. : CSC-RR0600 Host Cell: SU-DHL-4
| Cat. No. | CSC-RR0600 |
| Description | This cell line is engineered to stably express GFP reporter gene in SU-DHL-4 cells. |
| Gene | GFP |
| Host Cell | SU-DHL-4 |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Reporter Type | Fluorescent protein |
| Application | 1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
The researchers investigated the impact of Raji-derived extracellular vesicles (Evs) on lymphoma cells, with a focus on proliferation, invasion, autophagy, and apoptosis. Methods included Cell Counting Kit-8 (CCK-8) assays, Transwell experiments, microscopy, Western blotting, and flow cytometry. They analyzed miR-106a expression, downregulated it in Raji cells, and isolated resulting Evs (Evs-in-miR-106a). The binding relationship between miR-106a and Beclin1 was validated through RNA pull-down and dual-luciferase reporter gene assays. Overexpression of Beclin1 in SU-DHL-4 and Farage cells was performed, assessing autophagy and apoptosis in SU-DHL-4 cells. Tumorigenicity assays in nude mice validated the effects of Raji-Evs. Results demonstrated that Raji-Evs promoted lymphoma cell proliferation and invasion by upregulating miR-106a, which was reversed upon miR-106a knockdown. Furthermore, Raji-Evs-carried miR-106a targeted Beclin1, inhibiting autophagy and apoptosis, thus facilitating tumor growth in vivo. This study elucidated the mechanism by which Raji-Evs inhibit lymphoma cell autophagy and apoptosis while promoting cell growth via the miR-106a/Beclin1 axis.
Figure 1. Inhibition of autophagy and apoptosis in SU-DHL-4 cells via the miR-106a/Beclin1 axis was observed, employing TEM, GFP-mRFP-LC3, WB, flow cytometry, and RT-qPCR techniques. (Tang J, et al., 2022)
If similar studies are conducted, the utilization of our GFP Reporter Cell Line - SU-DHL-4 GFP reporter gene cell line would streamline the experimental procedures described. Within a unified cellular model, simultaneous monitoring of autophagy, apoptosis, and related protein expression levels can be achieved. This encompasses fluorescence microscopy for autophagic pathway observation, Western blot analysis for protein expression levels, and RT-qPCR for miRNA and mRNA expression analysis. These advancements simplify the assessment of cellular processes, thereby enhancing experimental efficiency and data coherence.
A: Stable transfection methods were utilized to integrate the GFP reporter gene into SU-DHL-4 cells, ensuring stable and sustained expression. Regular monitoring using fluorescence microscopy or flow cytometry confirmed stable GFP expression levels over time.
A: The SU-DHL-4 GFP Reporter Cell Line serves as a valuable tool for studying lymphoma biology, allowing visualization and tracking of SU-DHL-4 lymphoma cells. Its stable GFP expression enables studies on cell proliferation, migration, and response to treatments.
A: The SU-DHL-4 GFP Reporter Cell Line facilitates drug screening assays in lymphoma research, providing a reliable platform for evaluating the efficacy of potential therapeutics. Its stable GFP expression allows for quantitative analysis of drug effects on SU-DHL-4 cell behavior, aiding in the identification of novel treatment candidates.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
From studying tumor microenvironment interactions to evaluating treatment responses, this cell line serves as an invaluable resource for unraveling the complexities of lymphoma biology and developing novel therapeutic strategies. The GFP Reporter Cell Line consistently provides robust signals, ensuring reproducible results and accurate interpretation of experimental outcomes.
Canada
12/30/2022
The GFP Reporter Cell Line in SU-DHL-4 cells offers bright and clear GFP expression, enabling detailed visualization and tracking of cellular processes in my lymphoma research.
French
12/24/2023
With GFP fluorescence, this cell line facilitates comprehensive investigation of tumor growth, metastasis, and response to therapy, advancing our understanding of lymphoma biology. Its stable GFP expression simplifies experimental workflows, allowing for efficient data collection and analysis in lymphoma research.
French
01/13/2020
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.