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Cat.No. : AAV00442Z
Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml
Serotype: AAV Serotype 2 Storage: -80 ℃
| Cat. No. | AAV00442Z |
| Description | This virus is a reporter AAV with capsid engineering / modification. GFP AAV2-PPS particles contain engineered capsid derived from AAV serotype 2 (AAV2) which has insertion of peptides DSPAHPS at I587. The target cell type of this capsid engineered AAV is central nervous system (CNS). |
| Reporter | GFP |
| Serotype | AAV Serotype 2 |
| Target Gene | GFP |
| Application | 1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery. |
| Titer | Varies lot by lot, typically ≥1x10^12 GC/mL |
| Size | Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc. |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Frozen on dry ice |
| Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots. | |
| Endotoxin | Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance. |
| Purity | AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE. |
| Sterility | The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth. |
| Transducibility | Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities. |
| Empty vs. Full Capsids | Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods. |
A: Green fluorescent protein (GFP) and its homologs are widely used as fluorescent markers of gene expression and for determination of protein localization and motility in living cells.
A: The unique life cycle of adeno-associated virus (AAV) and its ability to infect non-dividing and dividing cells and to sustain expression make it an attractive vector. Another attractive feature of wild-type viruses is the lack of overt pathogenicity.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
The engineered capsid modification with the DSPAHPS peptide insertion at I587 ensures remarkable targeting precision, allowing us to achieve higher transduction rates in neuronal cells compared to other vectors we've used.
United States
01/07/2020
The engineered capsid of the GFP AAV2-PPS demonstrated superior delivery and penetration into the central nervous system. This capability greatly enhances the efficacy of our experimental protocols, making it an invaluable tool for CNS-related research.
French
11/22/2022
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