Cre-GFP Adeno-associated virus(AAV Serotype 2)

Name: Cre-GFP Adeno-associated virus(AAV Serotype 2)
SKU: AAV00062Z
Rating: 4.0 (1 reviews)

Cat.No. : AAV00062Z

Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml

Serotype: AAV Serotype 2 Storage: -80 ℃

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AAV Particle Information

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Gene Informationn

Cat. No.	AAV00062Z
Description	Cre-GFP Adeno-associated virus(AAV Serotype 2) expresses Cre recombinase and eGFP under the control CMV promoter in two separate expression cassettes. This product uses the Cre-lox system as a genetic tool to generate site-specific recombination of DNA between loxP sites in cultured cells and animal experiments.
Serotype	AAV Serotype 2
Target Gene	gfp
Product Type	Adeno-associated virus
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

Gene Name	gfp green fluorescent protein [ Neisseria gonorrhoeae ]
Gene Symbol	GFP
Synonyms	GFP; green fluorescent protein;
Gene ID	7011691

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Cre-GFP Adeno-associated virus (AAV serotype 2) is a widely used tool in molecular biology and genetic engineering to study gene function and regulation. This gene delivery vector combines Cre recombinase and green fluorescent protein (GFP) within the AAV2 capsid. Cre recombinase is an enzyme derived from P1 bacteriophage that recognizes loxP sites on DNA. When these sites are strategically placed in the genome of an organism, Cre can recombinate DNA at these sites, resulting in deletion, inversion, or translocation of specific genomic segments. This enables scientists to create conditional knockouts or express genes in a tissue-specific manner, thereby elucidating gene function in a variety of biological contexts.

On the other hand, GFP is a well-known marker used to visualize and track gene expression due to its inherent ability to fluoresce under specific lighting conditions. GFP fused to Cre recombinase in AAV vectors allows researchers to monitor the expression and activity of the recombinase in real time, providing a powerful tool for mapping gene expression patterns in vivo.

Grb2-associated binding protein 1 (Gab1) is a docking/scaffolding molecule known to play important roles in cell growth and survival. Here, researchers show that Gab1 is reduced in cholinergic neurons in Alzheimer's disease (AD) patients and AD mouse models. In mice, selective ablation of Gab1 in medial septal cholinergic neurons impairs learning and memory as well as hippocampal long-term potentiation. Gab1 ablation also inhibits SK channels, leading to increased firing in septal cholinergic neurons. On the other hand, Gab1 overexpression improves cognitive function and restores hippocampal CaMKII autophosphorylation in AD mice. These results suggest that Gab1 plays an important role in the pathophysiology of AD and may represent a novel therapeutic target for diseases involving cholinergic dysfunction.

In this study, recombinant AAV2-GFP vector encoding Cre recombinase (AAV2-GFP-Cre) or scrambled sequence AAV2 for control was injected into the septum of 8-week-old Gab1f/f mice. Gab1 expression in Cre-expressing neurons was significantly suppressed 3 weeks after injection (Figure 1A). The same injection had no effect on Gab2 expression (Figure 1B). Silencing Gab1 had no effect on grip strength, but it led to a significant decrease in the discrimination index in the NORT (Figure 1C) and a decrease in the success rate of the Y-maze test (Figure 1D).

Figure 1. Loss of Gab1 in the septum leads to cognitive dysfunction. (A) Distribution of AAV2-Cre-GFP in the septum of Gab1^f/f mice 3 weeks after microinjection. (B) Left: Representative Western blots of Gab1 and Gab2 in the septum of Gab1^f/f mice injected with AAV2-Cre-GFP and mice injected with scrambled sequence. Right: Gab1 protein expression in mice injected with scrambled sequence was quantified as a densitometry ratio. (C) NORT data of Gab1^f/f mice injected with AAV2-Cre-GFP and mice injected with scrambled sequence. (D) Y-maze data of Gab1^f/f mice injected with AAV2-Cre-GFP and mice injected with scrambled sequence. (Lu N N, et al., 2018)

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Customer Reviews

Excellent Support

The customer support and resources provided by the Creative Biogene are top-notch. Their knowledgeable team is always ready to assist with any technical concerns, and the availability of extensive documentation and online resources helps ensure successful applications.

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10/07/2022

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Cre-GFP Adeno-associated virus(AAV Serotype 2)

AAV Particle Information

Quality Control

Gene Informationn

Background

Case Study

Publications

Q & A

Customer Reviews

Excellent Support

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