AAV3b-CAG-GFP

Name: AAV3b-CAG-GFP
SKU: AAV00388Z
Rating: 4.0 (1 reviews)

Cat.No. : AAV00388Z

Titer: ≥1x10^12 GC/mL / ≥1x10^13 GC/mL Size: 30 ul/100 ul/500 ul/1 ml

Serotype: AAV Serotype 3 Storage: -80 ℃

Inquire for Price

AAV Particle Information

Quality Control

Cat. No.	AAV00388Z
Description	Premade AAV particles in serotype 3b (AAV 3b) express GFP reporter gene from the CAG promoter.
Reporter	GFP
Serotype	AAV Serotype 3
Target Gene	GFP
Application	1. Determination of optimal MOI (multiplicity of infection), administration methods etc. 2. Detection of the infection efficiency of the AAV serotype against a specific cell type or tissue. 3. Using reporter genes to visualize the distribution and expression of AAV vectors in live animals, helping assess the biodistribution and persistence of gene delivery.
Titer	Varies lot by lot, typically ≥1x10^12 GC/mL
Size	Varies lot by lot, for example, 30 μL, 50 μL, 100 μL etc.
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Frozen on dry ice

Creative Biogene ensures high-quality AAV particles by optimizing and standardizing production protocols and performing stringent quality control (QC). The specific QC experiments performed vary between AAV particle lots.
Endotoxin	Endotoxins, primarily derived from Gram-negative bacteria, can trigger adverse immune responses. Endotoxin contamination is a significant concern in the production of AAV, especially for applications in animal studies and gene therapy. Effective endotoxin quality control is essential in the development and manufacturing of AAV particles. Creative Biogene utilizes rigorous endotoxin detection methods to monitor the endotoxin level in our produced AAV particles to ensure regulatory compliance.
Purity	AAV purity is critical for ensuring the safety and efficacy of AAV-based applications.AAV capsids are composed of three main protein components, known as viral proteins: VP1, VP2, and VP3. These proteins play a critical role in the structure and functionality of the AAV capsid. Monitoring the VP1, VP2, and VP3 content in AAV preparations is essential for quality control in AAV production. Our AAV particles are tested for showing three clear bands of VP1, VP2 VP3 by SDS-PAGE.
Sterility	The AAV virus samples are inoculated into the cell culture medium for about 5 days to detect bacterial and fungal growth.
Transducibility	Upon requirement, Creative Biogene can perform in vitro or in vivo transduction assays to evaluate the ability of AAV to deliver genetic material into target cells or tissues, and assess gene expression and functional activities.
Empty vs. Full Capsids	Based-on our proprietary AAV production and purification technology, Creative Biogene can always offer AAV particles with high ratio of full capsids. If required, we can also assess the ratio for a specifc lot of AAV particles by transmission electron microscopy (TEM) or other methods.

Quick Inquiry

Background

Publications

Q & A

Customer Reviews

AAV was initially discovered as a contaminant in adenovirus preparations. The AAV genome consists of single-stranded DNA of approximately 4.8 kilobases (kb) in length. In addition, AAV has a small (~25 nm) icosahedral capsid composed of three structural proteins, namely VP1, VP2, and VP3. AAV is a replication-defective parvovirus that traditionally requires co-infection with a helper adenovirus or herpes virus for efficient infection. Currently, AAV-helper-free systems are mainly used in clinical research. AAV itself is considered non-pathogenic and has not been specifically associated with any known human disease.

There are at least 12 natural serotypes of AAV, each of which exhibits different tissue tropisms. This is primarily due to the different affinities of these serotypes for a range of primary cell surface glycoprotein receptors and secondary or co-receptors. For example, heparan sulfate proteoglycans are thought to be the primary receptor for AAV-2. Reported AAV coreceptors include alpha V beta5 integrin, fibroblast growth factor receptor 1 (FGFR-1), and hepatocyte growth factor receptor (c-Met). Attachment of AAV-3 strain H is dependent on heparin, heparan sulfate, and FGFR-1.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Customer Reviews

Consistent Quality

I appreciate the quality and consistency of Creative Biogene’s AAV3b-CAG-GFP vectors. They provide robust fluorescence, making it easy to track and analyze gene delivery in our experiments.

French

11/30/2024

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *

First Name

Country *

Email *

AAV3b-CAG-GFP

AAV Particle Information

Quality Control

Background

Publications

Q & A

Customer Reviews

Consistent Quality

Related Services