GFP Reporter Cell Line - OCI-AML-3

KU-812E is a well-established human cell line derived from a patient with chronic myeloid leukemia (CML). Due to its well-documented genetic and phenotypic characteristics, this cell line has played an important role in advancing our understanding of leukemia biology, drug resistance, and treatment response. KU-812E cells are derived from the peripheral blood of CML patients in blast crisis and possess typical properties of hematopoietic progenitors, such as expression of erythroid markers and differentiation capacity. In addition, KU-812E cells are frequently used in studies focused on mechanisms of drug resistance, providing insights into the molecular pathways that leukemic cells exploit to evade chemotherapy. Researchers frequently use these cells to identify new drug targets and develop combination therapies aimed at overcoming drug resistance and achieving better clinical outcomes for CML patients. GFP/Luc reporter cell lines, particularly the KU-812E variant, represent a major innovation in biomedical research, especially in studies involving gene expression, cell signaling, and cancer research. This reporter cell line is derived from the KU-812E human cell line, which was derived from a patient with chronic myeloid leukemia (CML) and has been genetically engineered to express green fluorescent protein (GFP) and luciferase (Luc) reporter genes. The GFP gene encodes a protein that fluoresces green under a specific wavelength of light, allowing for real-time, non-invasive visualization of cellular processes. This is particularly useful in live cell imaging, where researchers can track dynamic changes in gene expression, cellular localization, or protein-protein interactions without disturbing the cells. On the other hand, the luciferase reporter system provides a highly sensitive and quantitative measurement of gene expression. Luciferase catalyzes a luminescent reaction, and the intensity of the emitted light is directly related to the level of luciferase expression. This luminescence can be easily measured with a luminometer, providing a reliable method for high-throughput screening and real-time monitoring.