GFP Reporter Cell Line - Jeko-1

Name: GFP Reporter Cell Line - Jeko-1
SKU: CSC-RR0530
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RR0530

Host Cell : JeKo-1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

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Cat. No.	CSC-RR0530
Description	This cell line is engineered to stably overexpress GFP reporter gene. A20 is a murine lymphoma cell line, which has been widely used in laboratory research. This cell line is a useful tool for fluorescent tracking of A20 cells.
Target Gene	GFP
Host Cell	JeKo-1
Host Cell Species	Homo sapiens (Human)
Reporter Type	Fluorescent protein
Applications	1. Gene expression studies 2. Protein localization 3. Drug screening and toxicology 4. Live cell imaging
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

CSC-RR0530-GFP Reporter Cell Line - Jeko-1-Datasheet

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Background

Applications

Q & A

Customer Reviews

The Jeko-1 cell line is a human chronic lymphocytic leukemia (CLL) cell line that is used for studying the biology of CLL and for drug development. The stable expression of the GFP reporter gene in Jeko-1 cells allows for the visualization of cellular processes, such as cell division, apoptosis, and the response to potential therapeutic agents. GFP serves as a non-invasive marker that can be observed in living cells, providing real-time information on the behavior of CLL cells.

The GFP reporter cell line in Jeko-1 cells is a valuable tool for studying the molecular mechanisms underlying CLL and for evaluating the efficacy of potential therapeutic agents. It enables researchers to track the behavior of leukemia cells in real-time, providing insights into the biology of this disease and the development of targeted therapies.

The Jeko-1 GFP Reporter Cell Line is a human chronic lymphocytic leukemia cell line that expresses GFP. This cell line is valuable for studying the molecular mechanisms of leukemia and for the development of novel therapeutic strategies.

(1)Leukemia Cell Biology: The Jeko-1 GFP cells can be used to investigate the biology of chronic lymphocytic leukemia, including the characterization of leukemic stem cells and the investigation of molecular pathways involved in disease progression.

(2)Drug Efficacy and Resistance: The GFP reporter allows for the assessment of the effects of various anti-leukemic drugs on cell viability and proliferation. Researchers can use this cell line to study the development of drug resistance and to identify potential therapeutic targets.

(3)Gene Function Studies: The Jeko-1 GFP cell line can be employed to study the function of genes that are implicated in leukemia. By manipulating gene expression and observing changes in GFP fluorescence, researchers can gain insights into gene function and its role in disease.

Customer Q&As

What are the optimal growth conditions for the GFP Reporter Cell Line - Jeko-1?

A: The optimal growth conditions for the GFP Reporter Cell Line - Jeko-1 involve culturing cells in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. Incubation at 37°C in a humidified atmosphere containing 5% CO2 is recommended for robust cell growth and GFP expression.

How can I validate the GFP expression in the GFP Reporter Cell Line - Jeko-1?

A: To validate GFP expression, you can perform fluorescence microscopy or flow cytometry analysis. For microscopy, visualize GFP fluorescence using appropriate filters. For flow cytometry, harvest cells, wash them, and analyze GFP fluorescence using a flow cytometer. Additionally, you can quantify GFP expression by measuring fluorescence intensity or by conducting Western blot analysis.

What methods can be employed to assess the stability of GFP expression in the GFP Reporter Cell Line - Jeko-1 over time?

A: To assess the stability of GFP expression, regularly passage the cell line over several generations and monitor GFP fluorescence. Measure GFP expression levels using fluorescence microscopy or flow cytometry at different passages. Additionally, perform stability assays such as long-term culture or drug treatments to evaluate GFP expression retention under various conditions.

How can I optimize transfection efficiency in the GFP Reporter Cell Line - Jeko-1?

A: To optimize transfection efficiency, consider using different transfection reagents and methods such as lipid-based transfection reagents or electroporation. Optimize transfection conditions including DNA concentration, cell density, and transfection reagent-to-DNA ratio. Additionally, pre-treat cells with compounds that enhance transfection efficiency or co-transfect with selectable markers to enrich for transfected cells.

What are potential applications of the GFP Reporter Cell Line - Jeko-1 in cancer research?

A: The GFP Reporter Cell Line - Jeko-1 can be utilized in various cancer research applications such as studying cancer cell proliferation, migration, and invasion. It can also be used to investigate signaling pathways involved in cancer progression or to screen for potential anticancer drugs. Furthermore, this cell line can facilitate the visualization and tracking of cancer cells in vivo in animal models, providing insights into tumor growth and metastasis.

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Customer Reviews

Clear GFP Expression

This cell line exhibits clear GFP expression, facilitating easy visualization and identification of transduced cells under fluorescence microscopy.

Canada

2023-11-04

Stable GFP Integration

GFP integration in the GFP Reporter Cell Line - Jeko-1 is stable, guaranteeing reliable and sustained GFP expression over time and passages.

French

2022-10-03

Compatible with Flow Cytometry

The GFP Reporter Cell Line - Jeko-1 is compatible with flow cytometry analysis, enabling quantification and sorting of GFP-expressing cells for downstream applications.

Canada

2020-01-17

Lymphoma Model Specificity

GFP Reporter Cell Line - Jeko-1 is derived from mantle cell lymphoma, providing a specific model for studying this type of cancer and related cellular processes.

Canada

2021-09-23

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GFP Reporter Cell Line - Jeko-1

Cell Line Information

Cell Culture Information

Safety and Packaging

Documents

Background

Applications

Q & A

Customer Reviews

What are the optimal growth conditions for the GFP Reporter Cell Line - Jeko-1?

How can I validate the GFP expression in the GFP Reporter Cell Line - Jeko-1?

What methods can be employed to assess the stability of GFP expression in the GFP Reporter Cell Line - Jeko-1 over time?

How can I optimize transfection efficiency in the GFP Reporter Cell Line - Jeko-1?

What are potential applications of the GFP Reporter Cell Line - Jeko-1 in cancer research?

Clear GFP Expression

Stable GFP Integration

Compatible with Flow Cytometry

Lymphoma Model Specificity

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