Tth DNA Polymerase

Name: Tth DNA Polymerase
SKU: EMDT0346

For research use only. Not intended for any clinical use.

Cat.No.

EMDT0346

Description

Minimizes problems with strong secondary structure of RNA

Applications

Can reverse transcribe at elevated temperatures. Minimizes problems with strong secondary structure of RNA. Results in greater specificity of primer hybridization and extension. Suitable for high temperature synthesis of DNA. Synthesizes cDNA from RNA template. Stable thermophilic DNA polymerase.

Concentration

5-10 units/μl

Reaction Buffer

25 mM Tris-HCl (pH 9.5 at 25 °C).50 mM potassium chloride.10 mM magnesium chloride.1 mM dithiothreitol.0.3 μg/μl activated calf thymus DNA.1 μg/μl bovine serum albumin.0.2 mM of each dCTP, dGTP, dTTP and [α-32P]dATP.Incubation is for 10 minutes at 70 °C in a total reaction volume of 50 μl.

Reaction Conditions

Incubation at 70 °C

Size/Form

5000 Units;1000 Units

Source

Thermus thermophilus lll

Storage

50 mM Tris HCl (pH 7.5) 0.1 mM EDTA5.0 mM dithiothreitol50% (v/v) glycerol Stabilizers

Unit Definition

One unit is the amount of enzyme required to incorporate 10 nmoles total nucleotide into acid insoluble material at 70 °C for 30 minutes.

Publications

Q & A

Customer Reviews

Customer Q&As

What are the advantages of the Tth DNA Polymerase?

A: The Tth DNA Polymerase has high purity (SDS-PAGE ≥ 95%); no exonuclease, DNase, RNase residues; high heat stability up to 95°C; and a PCR amplification detection sensitivity of up to 100 pg.

Can it be used to amplify complex templates?

A: In the presence of Mn2+, the enzyme exhibits strong reverse transcriptase activity at 55-70°C, is compatible with complex templates, and has no RNase H activity, showing excellent performance in one-step RT-PCR reactions.

What is the enzymatic activity?

A: Tth DNA polymerase is a heat-resistant DNA polymerase discovered from Thermus thermophilus HB8 and has 5′-3′ DNA polymerase activity and 5′-3′ exonuclease activity in the presence of Mg2+, with no 3′-5′ exonuclease activity. It can be widely used in PCR amplification reactions.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

First Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *