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Constitutive Reporter Cell Lines offered by Creative Biogene provide a diverse selection of cell types suitable for a wide range of research applications. These cell lines are engineered to stably express fluorescent proteins (GFP or RFP) or luciferase reporters, enabling continuous monitoring of cellular processes without the need for exogenous induction. With Creative Biogene's Constitutive Reporter Cell Lines, researchers gain invaluable tools to explore cellular biology, accelerate drug discovery efforts, and advance scientific knowledge across various fields.
Key Features and Advantages
Illuminate your research with Creative Biogene's Fluorescent Reporter Cell Line, providing vibrant and real-time insights into cellular dynamics.
Shed light on cellular pathways with Creative Biogene's Bioluminescent Reporter Cell Line, offering sensitive and reliable monitoring of biological activities.
Double your investigative power with Creative Biogene's Dual Reporter Cell Line, combining fluorescence and bioluminescence for comprehensive cellular analysis.
Reporter genes are vital tools in gene expression studies, widely used across biomedicine, pharmaceuticals, molecular biology, and biochemistry. Typically fused with regulatory regions, they form operon fusions, ensuring their expression mirrors that of natural genes. By linking promoter sequences with easily detectable reporter genes like firefly luciferase, researchers can measure the regulatory potential of unknown DNA sequences, tracking and studying genes in various organisms and cell cultures. Constitutive Reporter Cell Line, a common reporter gene system, offers sensitivity, reliability, and scalability for large-scale gene expression studies, aiding in understanding molecular events and disease mechanisms.
Applications
Case Study 1
Due to its location as the outermost layer of the body, human skin is consistently subjected to a variety of stressors. Researchers aimed to investigate the impact of Clematis apiifolia DC. extract (CAE) on B[a]P-induced effects in HaCaT human keratinocytes. They observed that CAE inhibited aryl hydrocarbon receptor (AhR) signaling, reducing B[a]P-induced cytotoxicity and carcinogenic effects. Additionally, CAE activated antioxidant response element (ARE) signaling, enhancing the expression of antioxidant genes. These findings suggest that CAE could potentially protect against the harmful effects of B[a]P-containing pollutants by suppressing AhR signaling and activating Nrf2-mediated antioxidant pathways.
Figure 1. Researchers utilized cell line reports to investigate the impact of CAE on ARE signaling, demonstrating Nrf2's involvement. The HEK293-TRPV1-luciferase stable cell line (from Creative Biogene Biotechnology, Shirley, NY, USA) was cultured in DMEM with 10% FBS, 10% puromycin, and 1% antibiotics at 37°C in a 5% CO2 humidified incubator. (Lee SE, et al., 2019)
Case Study 2
Despite ICG fluorescence imaging's use in detecting lung cancer, consensus on optimizing ICG injection remains lacking. Researchers endeavored to refine the administration of indocyanine green (ICG) for detecting lung cancer, utilizing both animal models and clinical observations. Preclinical investigations involving mice and rabbits explored various ICG doses and timings, culminating in the identification of an optimal regimen: 2 mg/kg ICG injected 12 hours prior to surgery. Clinical trials confirmed the efficacy of this approach, successfully detecting 37 out of 39 cases with a consolidation-to-tumor (C/T) ratio >50%. Nevertheless, there remains a necessity for further exploration to devise enhanced fluorescent agents specifically targeting lung cancer, particularly for cases with a C/T ratio ≤50%.
Figure 2. Time and dose optimization of ICG for tumor detection in mice model with footpad tumor was conducted by researchers, aiming to enhance NIR fluorescence imaging and quantification of tumor-specific signals. LLC-GFP (Lewis lung carcinoma–green fluorescent protein) cell lines from Creative Biogene, Shirley, NY, USA, were utilized to develop mouse models of footpad cancer. (Jeon OH, et al., 2024)
Q: How can I ensure the stability and consistency of the Fluorescent reporter cell line during usage?
A: To ensure the stability and consistency of the Fluorescent reporter cell line, we recommend taking the following steps:
(1) Regularly validate the cell line to confirm stable expression of the reporter gene and check cell morphology.
(2) Strictly control cell culture conditions, including temperature, CO2 concentration, and culture medium components, to maintain stability.
(3) Avoid excessive passaging and use of aged cell lines, and regularly refresh the cell line to maintain stability.
(4) Maintain consistency in experimental design and procedures to minimize experimental errors.
Q: How can I distinguish between real signals and background signals during fluorescence signal detection?
A: To differentiate between real signals and background signals, the following approaches can be employed:
(1) Design appropriate control experimental groups to detect and eliminate the influence of background signals.
(2) Use high-quality fluorescent probes and filters to minimize background signal interference.
(3) Regularly inspect experimental instruments to ensure stable performance and reduce background signal sources' impact.
(4) Utilize suitable data processing methods, such as background correction or signal smoothing, to enhance the accuracy and reliability of signal detection.
Q: How can I ensure that the fluorescence signal intensity of the Fluorescent reporter cell line is sufficient for experimental analysis?
A: To ensure adequate fluorescence signal intensity, the following strategies can be employed:
(1) Utilize Fluorescent reporter cell lines with high expression levels or enhance their expression levels through gene editing techniques.
(2) Optimize fluorescent probes and detection methods to increase the sensitivity and resolution of signal detection.
(3) Pay attention to adjustments in cell culture conditions to ensure cells are in optimal states, thereby enhancing fluorescence signal intensity and stability.
Q: How can I address potential background interference issues during experiments?
A: To address background interference issues, the following measures can be taken:
(1) Optimize cell culture conditions to minimize intracellular and extracellular interference substances.
(2) Employ background correction or removal methods to eliminate background signal interference.
(3) Select appropriate experimental timing and conditions to minimize the influence of external light sources or other interference factors.
(4) Regularly inspect experimental equipment to ensure normal operation and eliminate potential sources of interference.
Q: How can I deal with cell line mutations or gene inactivation during experiments?
A: To address cell line mutations or gene inactivation, the following strategies can be implemented:
(1) Regularly validate cell lines to promptly detect and address mutations or inactivation phenomena.
(2) Optimize cell culture conditions to improve cell survival and stability.
(3) Use cell protectants and antioxidants to reduce cell damage and mutation risks.
(4) Strictly control conditions during experimental design and procedures to minimize adverse effects on cells.
| Cat.No. | Product Name | Price |
|---|---|---|
| CSC-RR0039 | CFP Reporter Stable Cell Line-HEK293 | Inquiry |
| CSC-RR0040 | GFP Reporter Stable Cell Line-HEK293 | Inquiry |
| CSC-RR0041 | luciferase Reporter Stable Cell Line-HEK293 | Inquiry |
| CSC-RR0042 | YFP Reporter Stable Cell Line-HEK293 | Inquiry |
| CSC-RR0043 | GFP-Puro Reporter Stable Cell Line-HEK293T | Inquiry |
| CSC-RR0045 | RFP Reporter Stable Cell Line-BT-549 | Inquiry |
| CSC-RR0046 | GFP Reporter Stable Cell Line-ES-2 | Inquiry |
| CSC-RR0047 | GFP Reporter Stable Cell Line-HeLa | Inquiry |
| CSC-RR0049 | Luciferase Reporter Stable Cell Line-MCF-7 | Inquiry |
| CSC-RR0057 | GFP Reporter Stable Cell Line-NIH3T3 | Inquiry |
| CSC-RR0058 | GFP Reporter Stable Cell Line-OVCA429 | Inquiry |
| CSC-RR0059 | RFP Reporter Stable Cell Line-OVCAR-5 | Inquiry |
| CSC-RR0060 | GFP/Luciferase Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR0061 | Luciferase Reporter Stable Cell Line - SK-OV-3 | Inquiry |
| CSC-RR00610 | Luciferase Reporter Cell Line - Farage | Inquiry |
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