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Human DNA Polymerase Gamma

For research use only. Not intended for any clinical use.
Cat.No.
EMDT0319
Description
Exclusively from CHIMERx, Human DNA Polymerase Gamma is a mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions
Applications
Mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions and testing replication of mitochondrial DNA.
Reaction Buffer
50 mM Tris-HCl (pH 8.7)10 mM MgCl20.4 mg/ml bovine serum albumin100 mM KCl15% glycerol0.05 mM of each dCTP, dGTP, dTTP, and [α-32P]dATP.10 μg activated DNA.Incubation for 15 minutes at 37 °C in a total reaction volume of 50 μl.
Reaction Conditions
Incubation at 37 °C
Size/Form
100 Units;50 Units
Storage
20 mM Tris-HCl (pH 8.0) 1.0 mM dithiothrietol0.1 mM EDTA0.2 M NaCl50% (v/v) glycerol
Unit Definition
One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37 °C.

Publications

Q & A

Customer Reviews

Customer Q&As
What are the reaction conditions for this enzyme?

A: The total reaction volume is 50 μl, and it is incubated at 37°C for 15 minutes.

Where does it come from?

A: Human DNA Polymerase Gamma exclusively from CHIMERx.

What are the different types of packaging for this product?

A: There are two types of packaging: 100 Units and 50 Units.

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Customer Reviews
Drug testing

We have successfully tested the toxicity of relevant drugs on key human oxidative functions using this reagent.

United States

05/05/2023

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