Exclusively from CHIMERx, Human DNA Polymerase Gamma is a mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions
Mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions and testing replication of mitochondrial DNA.
50 mM Tris-HCl (pH 8.7)10 mM MgCl20.4 mg/ml bovine serum albumin100 mM KCl15% glycerol0.05 mM of each dCTP, dGTP, dTTP, and [α-32P]dATP.10 μg activated DNA.Incubation for 15 minutes at 37 °C in a total reaction volume of 50 μl.
Incubation at 37 °C
100 Units;50 Units
20 mM Tris-HCl (pH 8.0) 1.0 mM dithiothrietol0.1 mM EDTA0.2 M NaCl50% (v/v) glycerol
One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37 °C.