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Cat. No. : EMDT0319
| Cat. No. | EMDT0319 |
| Description | Exclusively from CHIMERx, Human DNA Polymerase Gamma is a mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions |
| Applications | Mitochondrial enzyme used for testing the toxicity of drugs on critical human oxidative functions and testing replication of mitochondrial DNA. |
| Size | 100 Units;50 Units |
| Unit Definition | One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37 °C. |
| Reaction Buffer | 50 mM Tris-HCl (pH 8.7)10 mM MgCl20.4 mg/ml bovine serum albumin100 mM KCl15% glycerol0.05 mM of each dCTP, dGTP, dTTP, and [α-32P]dATP.10 μg activated DNA.Incubation for 15 minutes at 37 °C in a total reaction volume of 50 μl. |
| Reaction Conditions | Incubation at 37 °C |
| Storage | 20 mM Tris-HCl (pH 8.0) 1.0 mM dithiothrietol0.1 mM EDTA0.2 M NaCl50% (v/v) glycerol |
A: The total reaction volume is 50 μl, and it is incubated at 37°C for 15 minutes.
A: Human DNA Polymerase Gamma exclusively from CHIMERx.
A: There are two types of packaging: 100 Units and 50 Units.
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We have successfully tested the toxicity of relevant drugs on key human oxidative functions using this reagent.
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