Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : EMDT0317
| Cat. No. | EMDT0317 |
| Description | The simplest DNA Polymerase known in both size and catalysis.Replication enzyme involved in initiation of DNA replication in eukaryotes.A multi-subunit complex, which inhibits DNA polymerase and primase activities.Isolated from HeLa cells by immunoaffinity chromatography |
| Applications | Used to test in vitro replication capabilities along with other enzymes.Used to test the toxicity of drugs on key human replication systems.Used in DNA replication experiments. |
| Size | 100 Units; 50 Units |
| Unit Definition | One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37 °C |
| Reaction Buffer | 60 mM Tris-HCl (pH 8.0). 5.0 mM magnesium acetate. 0.3 mg/ml bovine serum albumin. 1.0 mM dithiothreitol. 0.1 mM spermine. 0.2 mM of each dCTP, dGTP, dTTP, dATP (pH 7.0). 0.1 mM [α-3H] dATP. 20 μg activated calf thymus DNA. Incubation at 37°C for 30 minutes in a total reaction volume of 30 μl. |
| Reaction Conditions | Incubation at 37 °C |
| Storage | 20 mM Tris-HCl (pH 8.0) 0.25 mM EDTA 50 mM NaCl 1 mM β-mercaptoethanol 0.1% Triton X-100 50% (v/v) glycerol |
A: Yes, its enzyme activity will not be inhibited by other enzymes.
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It is isolated from HeLa cells by immunoaffinity chromatography and has a high purity.
It only needs to be incubated at 37℃ for 30 minutes and has high enzyme activity.
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