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Human DNA Polymerase Alpha

For research use only. Not intended for any clinical use.

Cat. No. :   EMDT0317

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Product Information

Cat. No. EMDT0317
Description The simplest DNA Polymerase known in both size and catalysis.Replication enzyme involved in initiation of DNA replication in eukaryotes.A multi-subunit complex, which inhibits DNA polymerase and primase activities.Isolated from HeLa cells by immunoaffinity chromatography
Applications Used to test in vitro replication capabilities along with other enzymes.Used to test the toxicity of drugs on key human replication systems.Used in DNA replication experiments.
Size 100 Units; 50 Units
Unit Definition One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37 °C
Reaction Buffer 60 mM Tris-HCl (pH 8.0). 5.0 mM magnesium acetate. 0.3 mg/ml bovine serum albumin. 1.0 mM dithiothreitol. 0.1 mM spermine. 0.2 mM of each dCTP, dGTP, dTTP, dATP (pH 7.0). 0.1 mM [α-3H] dATP. 20 μg activated calf thymus DNA. Incubation at 37°C for 30 minutes in a total reaction volume of 30 μl.
Reaction Conditions Incubation at 37 °C
Storage 20 mM Tris-HCl (pH 8.0) 0.25 mM EDTA 50 mM NaCl 1 mM β-mercaptoethanol 0.1% Triton X-100 50% (v/v) glycerol
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Can it be used with other DNA polymerases?

A: Yes, its enzyme activity will not be inhibited by other enzymes.

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Customer Reviews
High purity and no contamination.

It is isolated from HeLa cells by immunoaffinity chromatography and has a high purity.

United States

The reaction conditions are simple and efficient.

It only needs to be incubated at 37℃ for 30 minutes and has high enzyme activity.

United States

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