Manta 1.0 DNA Polymerase

EMDT0324

Manta 1.0 DNA Polymerase (exo-) is a thermophilic DNA polymerase deficient in both proofreading (3′→ 5′) and nick-translation (5′→ 3′) nuclease activities. The protein was originally characterized and its crystal structure solved by Lorena Beese.

400000 U/ml

200 mM Tris-HCl 100 mM (NH₄)₂SO₄ 100 mM KCl 20 mM MgSO 41.0% Triton X-100 pH 8.8 @ 25 °C

100000 Units

A recombinant E. coli strain carrying the Manta 1.0 DNA Polymerase (exo-) polymerase gene.

10 mM Tris-HCl 50 mM KCl 1.0 mM DTT 0.1 mM EDTA 0.1% Triton X-100 50% glycerol pH 7.5 at 25 °C

A recombinant E. coli strain carrying the Manta 1.0 DNA Polymerase (exo-) polymerase gene.