Manta 1.0 DNA Polymerase

Name: Manta 1.0 DNA Polymerase
SKU: EMDT0324
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMDT0324

Inquire for Price

Product Information

Cat. No.	EMDT0324
Description	Manta 1.0 DNA Polymerase (exo-) is a thermophilic DNA polymerase deficient in both proofreading (3′→ 5′) and nick-translation (5′→ 3′) nuclease activities. The protein was originally characterized and its crystal structure solved by Lorena Beese.
Source	A recombinant E. coli strain carrying the Manta 1.0 DNA Polymerase (exo-) polymerase gene.
Concentration	400000 U/ml
Size	100000 Units
Unit Definition	A recombinant E. coli strain carrying the Manta 1.0 DNA Polymerase (exo-) polymerase gene.
Reaction Buffer	200 mM Tris-HCl 100 mM (NH₄)₂SO₄100 mM KCl 20 mM MgSO 41.0% Triton X-100 pH 8.8 @ 25 °C
Storage	10 mM Tris-HCl 50 mM KCl 1.0 mM DTT 0.1 mM EDTA 0.1% Triton X-100 50% glycerol pH 7.5 at 25 °C

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

How should Manta 1.0 DNA Polymerase be stored?

A: The Manta 1.0 DNA Polymerase should be stored at -25°C to -15°C, and it is recommended to keep it on ice during use.

What is the source of the recombinant enzyme protein?

A: The recombinant E. coli strain carrying the Manta 1.0 DNA polymerase (exo-) gene produces the enzyme.

Does the Manta 1.0 DNA Polymerase have endonuclease activity?

A: No, Manta 1.0 DNA Polymerase does not have 5'→3' or 3'→5' exonuclease activity.

How is the unit of the Manta 1.0 DNA Polymerase defined?

A: One unit (U) is defined as the amount of enzyme that catalyzes the incorporation of 65 nmol of dNTP into acid-insoluble material in 30 minutes at 10°C.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *