Taq DNA polymerase

Name: Taq DNA polymerase
SKU: EMQZ1407
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : EMQZ1407

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Product Information

Cat. No.	EMQZ1407
Description	Taq DNA Polymerase (regular) is a thermostable enzyme with a highly processive 5''->3'' polymerase activity and 5''->3'' exonuclease activity. The enzyme is purified from E.coli and consists of a single polypeptide with a molecular weight of 94 kDa. Taq DNA polymerase synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.
Source	A recombinant E. coli strain that carries the cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.
Concentration	5,000 U/ml
Applications	• PCR (ordinary and high-throughput) • Primer Extension • Microarray Analysis • Denaturing high performance liquid chromatography (DHPLC)
Size	400 U; 2,000 U
Unit Definition	One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 72°C.
Reaction Conditions	100 ul reaction system containing: 1×standard Taq Reaction Buffer [10 mM Tris-HCl (pH 8.3 at 25 °C),50 mM KCl,1.5 mM MgCl2]; DNA template,primers,200 µM dNTPs and 2-5 U DNA polymerase.
Storage	Store at -20°C

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Customer Reviews

Customer Q&As

What is the amplification efficiency of the enzyme?

A: When used, dilute it to 1×, and its amplification speed is 1kb/min.

What is the typical number of cycles used when performing cDNA amplification?

A: It is recommended to run 30-35 cycles for template amplification, while up to 40 cycles for bacterial PCR.

How long does it take to receive the goods after purchase?

A: If there is stock available, it can be delivered within 3-5 days. If there is no stock available, it should not take more than a week for delivery.

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Customer Reviews

High cost performance

When used for bacterial PCR, the amplification effect is good and cheap.

United States

2023-03-31

Easy product connection

The PCR product of Taq DNA polymerase will have an extra A at the 3' end, which can be ligated to a complementary T on the plasmid.

United States

2021-01-02

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Taq DNA polymerase

Product Information

Q & A

Customer Reviews

What is the amplification efficiency of the enzyme?

What is the typical number of cycles used when performing cDNA amplification?

How long does it take to receive the goods after purchase?

High cost performance

Easy product connection

Related Products

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