Taq DNA polymerase

EMQZ1407

Taq DNA Polymerase (regular) is a thermostable enzyme with a highly processive 5''->3'' polymerase activity and 5''->3'' exonuclease activity. The enzyme is purified from E.coli and consists of a single polypeptide with a molecular weight of 94 kDa. Taq DNA polymerase synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

• PCR (ordinary and high-throughput) • Primer Extension • Microarray Analysis • Denaturing high performance liquid chromatography (DHPLC)

5,000 U/ml

100 ul reaction system containing: 1×standard Taq Reaction Buffer [10 mM Tris-HCl (pH 8.3 at 25 °C),50 mM KCl,1.5 mM MgCl2]; DNA template,primers,200 µM dNTPs and 2-5 U DNA polymerase.

400 U; 2,000 U

A recombinant E. coli strain that carries the cloned Taq DNA Polymerase gene from Thermus aquaticus YT-1.

Store at -20°C

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 72°C.