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pComb3H represents the second generation of the pComb vector series, featuring enhancements in stability and the incorporation of new cloning regions utilizing asymmetric SfiI restriction sites for directional cloning. This enables the cloning of intact Fabs, scFvs, peptides, and other proteins for phage display. The vector includes the bacterial ampicillin resistance gene, ensuring only transformed bacteria grow during phage production. pComb3H facilitates the efficient cloning of diverse antibody-encoding genes into phagemid vectors, enabling the generation of a diverse repertoire of antibodies with unique antigen-binding properties. Additionally, it allows for the construction of scFv and Fab fragment libraries, broadening the scope and potential of antibody libraries.
Key Features of Our pComb3H Set
The pComb3H Set is derived from phagemid pComb3, one of the pioneering phage display vectors used for generating and selecting antibody libraries in 50-kDa Fab format. Phagemids are plasmids containing the origin of replication and packaging signals for filamentous phage. pComb3H has been optimized for the expression of human fragments, particularly for proteins like zinc finger proteins and antibody fragments, which are displayed on phage pili for phage display selection. pComb3H set offers a versatile platform for various applications in molecular biology and biotechnology. Some key application areas include:
Case Study 1
Super-resolution microscopy is advancing virology and microbiology by mapping molecular assemblies critical for host-pathogen interfaces below the resolution limit of light microscopy. Researchers employed the pComb3H method to gain structural and dynamic insights into HIV-1 assembly using simultaneous dual-color superresolution microscopy. By reconstructing single virus assembly sites with live-cell PALM, they assessed the sub-viral mobility of the HIV-1 envelope glycoprotein during interaction with the viral lattice. This approach allows for the quantification of pathogen and host protein-protein interaction dynamics at the nanoscale, enhancing understanding of molecular assemblies critical for host-pathogen interfaces below the resolution limit of conventional light microscopy.
Figure 1. Researchers utilized the pComb3H system to produce BG18-QD625. This system enabled the incorporation of an unnatural amino acid (UAA) into the BG18 fab, facilitating noncanonical expression for subsequent quantum dot click reaction and purification via a His-(×6) tag. (Groves NS, et al., 2020)
Case Study 2
The SARS-CoV-2 pandemic poses a global health threat. Understanding how human antibodies respond to the virus could offer crucial insights for both research and clinical diagnosis. Researchers aimed to develop a diagnostic tool for SARS-CoV-2 infection using pComb3H. They constructed a Fab antibody phage library from COVID-19 patients' peripheral blood and identified 159 Fab antibodies by panning with NP. Subsequently, they evaluated 16 antibodies for binding properties and epitope recognition. Finally, two well-paired antibodies were selected for SARS-CoV-2 diagnosis using a double-antibody sandwich ELISA method. This approach may offer a valuable resource for rapid and accurate clinical diagnosis of SARS-CoV-2 infection.
Figure 2. Light chain genes were cloned into the phagemid vector pComb3H between Xab I and SacI restriction sites, forming the light chain gene pool. Subsequently, the heavy chain genes were sequentially cloned into the light chain pool in pComb3H between Xho I and Spe I restriction sites according to a previously published protocol. (Zhang L, et al., 2020)
Q: What makes the pComb3H Set stand out from other phage display vector systems available in the market?
A: Creative Biogene's pComb3H Set offers several advantages over other phage display vector systems. Firstly, it is derived from the well-established pComb3 vector, known for its reliability and efficiency in antibody library construction. Additionally, the pComb3H Set has been optimized for the expression of human fragments, ensuring high levels of protein expression and display on phage pili. Our rigorous quality control measures further ensure the reliability and consistency of the pComb3H Set, making it a preferred choice for researchers in the field.
Q: How does Creative Biogene ensure the quality and integrity of the pComb3H Set products?
A: Creative Biogene places a strong emphasis on product quality and integrity. Our pComb3H Set undergoes stringent quality control processes at every stage of production, from vector construction to final packaging. We use advanced molecular biology techniques and analytical methods to confirm the correct sequence, functionality, and purity of each vector in the set. Additionally, our products are shipped with detailed quality assurance documentation to provide customers with confidence in the reliability and performance of the pComb3H Set.
Q: Can you provide examples of successful applications or research studies using the pComb3H Set?
A: The pComb3H Set has been widely used in numerous research studies and applications across various fields of biotechnology and molecular biology. Researchers have utilized this vector set for the construction of diverse antibody libraries, protein engineering projects, and drug discovery initiatives. Many published studies highlight the effectiveness and versatility of the pComb3H Set in generating high-affinity binders, characterizing protein-protein interactions, and developing novel therapeutic molecules.
Q: How does Creative Biogene support customers in troubleshooting or optimizing their experiments using the pComb3H Set?
A: Creative Biogene provides comprehensive technical support to assist customers in troubleshooting or optimizing their experiments with the pComb3H Set. Our team of experienced scientists is available to address any technical inquiries, offer troubleshooting advice, and provide protocol optimization recommendations. Additionally, we offer customized services, such as vector modification or library construction, to meet specific experimental needs and ensure successful outcomes.
Q: What are the key features of the pComb3H Set that make it suitable for a wide range of phage display applications?
A: The pComb3H Set offers several key features that make it highly versatile and suitable for diverse phage display applications. These features include optimized expression of human fragments, efficient cloning regions for directional cloning of intact Fabs and scFvs, and compatibility with various display formats. Additionally, the vector set is designed for ease of use and flexibility, allowing researchers to tailor their experiments according to specific research goals and requirements.
| Cat.No. | Product Name | Price |
|---|---|---|
| VPT4016 | pComb3H vector | Inquiry |
| VPT4017 | pComb3HSS vector | Inquiry |
| VPT4018 | pComb3HTT vector | Inquiry |
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