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Cat. No. : EMDT0331
| Cat. No. | EMDT0331 |
| Description | E. coli derived polymerase, polyadenylating 3" termini of RNA |
| Source | Escherichia coli |
| Concentration | 1 Unit/μl |
| Applications | Adds poly A tails to the 3'-hydroxyl terminus of RNA. Labels 3'-ends of RNA with a [α-32P] cordycepin triphosphate. Ideal for determining molar concentration of polyadenylated RNA. |
| Size | 500 Units; 100 Units |
| Unit Definition | One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled ATP into acid insoluble form for 10 minutes at 37 °C under the stated assay conditions. |
| Reaction Buffer | 50 mM Tris-HCl (pH 7.9).10 mM MgCl2. 2.5 mM MnCl2. 250 mM NaCl. 25 μg tRNA. 0.1 mM [α-32P] ATP. 50 μg bovine serum albumin. Reaction volume of 100 μl. |
| Reaction Conditions | Incubation at 37 °C |
| Storage | 25mM Tris-HCl (pH 7.9) 0.1 mM DTT 0.01% (v/v) Triton X-100 1.0 mM EDTA 0.5M NaCl 50% (v/v) glycerol |
A: One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled ATP into acid-insoluble form in 10 minutes at 37°C under the specified assay conditions.
A: It catalyzes the sequential addition of ATP as AMP to the 3' end of RNA, thereby adding a poly(A) tail to the RNA.
A: It is used as a primer for single-stranded RNA. Double-stranded RNA, synthetic polyribonucleotides, and short oligoribonucleotides are not suitable as primers. DNA cannot be used as a primer.
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The purity of the Poly A Polymerase is 99% without any impurities that could affect experimental results.
The delivery cycle for Poly A Polymerase is 1-3 days, and it can generally be received within a week.
Polyadenylation enhances the stability of RNA in cells and improves the expression efficiency of RNA in eukaryotic cells after transfection or microinjection.
Poly A Polymerase has high tailing efficiency and can add 20-200 A bases to the 3' end of RNA.
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