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Poly A Polymerase

For research use only. Not intended for any clinical use.
Cat.No.
EMDT0331
Description
E. coli derived polymerase, polyadenylating 3" termini of RNA
Applications
Adds poly A tails to the 3'-hydroxyl terminus of RNA. Labels 3'-ends of RNA with a [α-32P] cordycepin triphosphate. Ideal for determining molar concentration of polyadenylated RNA.
Concentration
1 Unit/μl
Reaction Buffer
50 mM Tris-HCl (pH 7.9).10 mM MgCl2. 2.5 mM MnCl2. 250 mM NaCl. 25 μg tRNA. 0.1 mM [α-32P] ATP. 50 μg bovine serum albumin. Reaction volume of 100 μl.
Reaction Conditions
Incubation at 37 °C
Size/Form
500 Units; 100 Units
Source
Escherichia coli
Storage
25mM Tris-HCl (pH 7.9) 0.1 mM DTT 0.01% (v/v) Triton X-100 1.0 mM EDTA 0.5M NaCl 50% (v/v) glycerol
Unit Definition
One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled ATP into acid insoluble form for 10 minutes at 37 °C under the stated assay conditions.

Publications

Q & A

Customer Reviews

Customer Q&As
How is Poly A Polymerase activity defined?

A: One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled ATP into acid-insoluble form in 10 minutes at 37°C under the specified assay conditions.

What is the function of the Poly A Polymerase?

A: It catalyzes the sequential addition of ATP as AMP to the 3' end of RNA, thereby adding a poly(A) tail to the RNA.

How should primers be selected when adding a Poly(A) tail?

A: It is used as a primer for single-stranded RNA. Double-stranded RNA, synthetic polyribonucleotides, and short oligoribonucleotides are not suitable as primers. DNA cannot be used as a primer.

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Customer Reviews
High purity

The purity of the Poly A Polymerase is 99% without any impurities that could affect experimental results.

United Kingdom

08/15/2021

Fast delivery speed

The delivery cycle for Poly A Polymerase is 1-3 days, and it can generally be received within a week.

United States

10/09/2021

Improved RNA expression efficiency

Polyadenylation enhances the stability of RNA in cells and improves the expression efficiency of RNA in eukaryotic cells after transfection or microinjection.

United States

01/27/2022

Can add multiple A's

Poly A Polymerase has high tailing efficiency and can add 20-200 A bases to the 3' end of RNA.

United States

07/19/2022

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